Re: [ccp4bb] Off-topic: NMR and crystallography

lography in terms of data collection > (having crystals in the first place) and data processing? How about > membrane proteins? > > > > I would appreciate replies to the board, instead of off-board, to allow > for a good discussion. > > > > Thank you. > > &

Re: [ccp4bb] השב: Re: [ccp4bb] Curious electron density associated with Asp sidechain

Tony. > > --- > Dr Antony W Oliver > Senior Research Fellow > CR-UK DNA Repair Enzymes Group > Genome Damage and Stability Centre > Science Park Road > University of Sussex > Falmer, Brighton, BN1 9RQ > > email: > antony.oli...@sussex.ac.uk<http://mc/compose?to=antony.oli...@sussex.ac.uk> > tel (office): +44 (0)1273 678349 > tel (lab): +44 (0)1273 677512 > > -- *Kornelius Zeth* *Email: kornelius.z...@gmail.com* *Unidad de Biofisica (CSIC-UPV/EHU) Barrio Sarriena s/n 48940, Leioa, Vizcaya* *SPAIN*

Re: [ccp4bb] off-topic:a dataset from a polluted sample

m looking forward for your suggestions. > > Enclosed pleased find the the sequencing result. > > Thanks very much! > Best regards. > > -- > Tianlong Zhang > -- *Kornelius Zeth* *Unidad de Biofisica (CSIC-UPV/EHU) Barrio Sarriena s/n 48940, Leioa, Vizcaya* *SPAIN*

Re: [ccp4bb] PISA server

other difficulties. > > > > Regards > > Miri Hirshberg, PDBe > > > > > > On Sat, 26 Mar 2011, Daniel Bonsor wrote: > > > >> Is the PISA server having problems? I can not seem to upload any > >> structure. > >> > >> Thanks &

Re: [ccp4bb] PISA server

hanks > > Dan -- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany kornelius.z...@tuebingen.mpg.de Tel -49 7071 601 323 Fax -49 7071 601 349

[ccp4bb] Question regarding S-SAD

! Best wishes Kornelius -- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany kornelius.z...@tuebingen.mpg.de Tel -49 7071 601 323 Fax -49 7071 601 349

[ccp4bb] SUMMARY: ROSETTA for MR model generation

(Edward Snell) http://www.ysbl.york.ac.uk/~fei/balbes/index.html -- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany kornelius.z...@tuebingen.mpg.de Tel -49 7071 601 323 Fax -49 7071 601 349

[ccp4bb] ROSETTA for MR model generation

is 1.9 A. Crystals are extremely rare. I would be grateful for any hints and will send a summary of all personally sent comments to the list. Thank you and have a nice day Kornelius -- Kornelius Zeth Max Planck Institute for Developmental Biology

Re: [ccp4bb] precipitation of protein in elution buffer

rs without using > dynamic light scattrer? please suggest any reference which suggest protocols > to check the solubility. I would appreciate the help. > > Thanks in advance > > Peter -- Kornelius Zeth Max Planck Institute for Deve

Re: [ccp4bb] Lost my protein

ank you very much. > > Yan > > > > ------ Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany kornelius.z...@tuebingen.mpg.de Tel -49 7071 601 323 Fax -49 7071 601 349

[ccp4bb]

BlackBerry > http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Mobile1_042009 ------ Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany kornelius.z...

Re: [ccp4bb] Design Constructs

s Pharmaceuticals, Inc. > Four Valley Square > 512 Township Line Road > Blue Bell, PA 19422 > email: vnambood...@locuspharma.com > Ph: 215-358-2012 > Fax: 215-358-2020 -- Kornelius Zeth Max Planck Institute for Developmental

[ccp4bb] Job opening at Max Planck / Friedrich Miescher Institute Tübingen

), electron microscopy (home EM). We are seeking a highly motivated biology/chemistry/physics student who has ideally acquired one of the above techniques during his/her diploma/master thesis. The project is a collaboration between the groups of Drs Kornelius Zeth (protein structure) and Dmitri

[ccp4bb] asymmetric oligomers

, salt bridges) between the two chains, interface seems ok, stable, low B-factors). Any comments and suggestions are appreciated Best wishes and thanks! Kornelius ------ Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolut

Re: [ccp4bb] Protein Color

mation about what this color might > suggest about the protein or how I could analyze possible bound metals or > prosthetic groups using only a small amount of protein would be helpful. > > Matt -- Kornelius Zeth Max Planck Institute for Develo

Re: [ccp4bb] Anion binding sites in proteins

There is a number of 'defined' anions known from structures of halorhodopsin and the cores of coiled-coil proteins often show them as well. -- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spem

Re: [ccp4bb] Sequence of crystallised protein fragment

--- > > > > > > > > Matthew LH Chu > PhD Student > School of Pharmacy and Pharmaceutical Sciences > University of Manchester > ---

Re: [ccp4bb] Right terminal residues for constructs.

. > > > I will be greatful for the insights from the scientific community. > I dont want to do any Directed evolution approach. > > -- > S.Jayashankar > Research Student > Institute for Biophysical Chemistry > Hannover Medical School > Germany ---

Re: [ccp4bb] Setting drops with proteins that are hard to concentrate

intended solely for > > the use of the individual or entity named on this message. If you > > are not the intended recipient, and have received this message in > > error, please notify us immediately by reply e-mail and then delete > > it from your system. > >

[ccp4bb] POSTDOC POSITION

of three scientists from whom letters of recommendation can be obtained to: -- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany [EMAIL PROTECTED] Tel -49 7071 601 323

[ccp4bb] Thermofluor experiment

. -- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany [EMAIL PROTECTED] Tel -49 7071 601 323 Fax -49 7071 601 349

Re: [ccp4bb] Engineering disulfide bonds

ression (assuming good geometry of the cysteines)? > > > Thanks, > > Kendall > -- > Kendall W. Nettles, PhD > Assistant Professor > Department of Cancer Biology > The Scripps Research Institute > 5353 Parkside Dr. > Jupiter Fl 33458 --

Re: [ccp4bb] AW: [ccp4bb] peptides for cocrystallization

> > > > Shed those extra pounds with MSN and The Biggest Loser! Learn more. > <http://biggestloser.msn.com/> > -- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany [EMAIL PROTECTED] Tel -49 7071 601 323 Fax -49 7071 601 349

Re: [ccp4bb] Plz suggest me

> > > __ > Sent from Yahoo! Mail - a smarter inbox http://uk.mail.yahoo.com -- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany [EMAIL PROTECTED] Tel -49 7071 601 323 Fax -49 7071 601 349

Re: [ccp4bb] Malonate Crystals?

208 > lab: 847.467.4049 > cel: 773.608.9185 > email: [EMAIL PROTECTED] > *** -- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany [EMAIL PROTECTED] Tel -49 7071 601 323 Fax -49 7071 601 349

[ccp4bb] sealed tube estimations

the majority of list people is not affected. One of the important requirements for us is: We are a small team of crystallography people and only little additional manpower for maintenance is available. Best wishes and have a nice day Kornelius ------

Re: [ccp4bb] MAD screen pattern

Met is incoperated > in the protein. The screen pattern is decribed as below. > Anybady had the experiences about this? > > Best wish > > jaishin > > > ------ Kornelius Zeth Max Planck Institute for Developmental Biology D

Re: [ccp4bb] How to replace 2.45M K/Na phosphate buffer - 2

t; Piazza Botta, 10 > > 27100 Pavia > > Italia > > Tel. 0039 0382 986335/8/1 > > Facs 0039 0382 303673 > > > _ > > Make every IM count. Download Windows Live Messenger > and join the i’m Initiative now. It

[ccp4bb]

le to get heavy metal > derivatives and > > I read that MPD does chelate heavy metals. Did any of > you make > > similar experiences with xtals in MPD conditions? If so > did you find > > a good solution (introduction of selmet makes the > protein ustable) > > t

response to: very acidic protein crystallization

ion pattern of your protein ? Could there be some flexible regions the prevent crystallization ? ------ Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany [EMAIL PROTECTED