you can do a simple wavelength scan at the synchrotron of the protein solution frozen in a loop.
Best wishes Kornelius On Fri, 5 Sep 2008 12:21:29 -0400 Matthew Alan Bratkowski <[EMAIL PROTECTED]> wrote: > Hello. > > I am working with a protein that turns a yellowish-brown color when it is > concentrated to around 2 mg/ml or higher in a small volume (a few hundred > uL). I was wondering if the protein bound a metal or other prosthetic > group that would give it this color? The protein's color somewhat > resembles iron binding proteins, but there is no peak in the 400 nm range > that would suggest heme, and an iron sulfur cluster is not that likely > since there are only five cysteines in the protein. Proteins with > structures homologous to the one I am studying bind magnesium, but are not > know to bind other metals. Any information about what this color might > suggest about the protein or how I could analyze possible bound metals or > prosthetic groups using only a small amount of protein would be helpful. > > Matt ---------------------------------------------- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany [EMAIL PROTECTED] Tel -49 7071 601 323 Fax -49 7071 601 349
