ancy of the MSE that gives negative difference density peaks.
Mark
Quoting Kayashree M:
Dear users,
I had posted this question already but in a different context.
One of the Se-Met derivatised protein that we have solve is a
homodimer
(with 4 MET in the chains that crystallised) of which
Dear users, I had posted this question already but in a different context.One of the Se-Met derivatised protein that we have solve is a homodimer (with 4 MET in the chains that crystallised) of which one chain has 3 MSEresidues, while the other chain has only 2 MSE.Are there any such instances in P
Respected Sir,
Thank you for the efforts and nice depiction of the problem.
The Dimeric assembly that is solved and the one predicted
by PISA is depicted in the attachment. This is the arrangement
I wanted to describe. The protein overall appears like a "C".
Thanking you
With Regards
KavyaSince
submitted this assembly itself. and by mistake I mentioned most probably biological assemblysection while submitting the PDB, Which gave rise to all confusions.Thanking youWith RegardsM. Kavyashree-Eleanor Dodson wrote: -To: Kayashree M From: Eleanor Dodson Date: 10/20/2011 03:23PMCc
Thank you Sir for the suggestions. Hi,If, in your case, no possible asymmetric unit can contain A1-B2, then you deposit A1-B1 (or I suppose A2-B2...) and indicate to the PDB (like placing cards in the header cards) the operator to be used (and the subunit it applies to) in order to generate the mos
while PISA predicts A1-B2.Thank youWith ReagrdsKavya-CCP4 bulletin board wrote: -To: CCP4BB@JISCMAIL.AC.UKFrom: James Stroud Sent by: CCP4 bulletin board Date: 10/19/2011 10:41PMSubject: Re: [ccp4bb] Biological assemblyOn Oct 19, 2011, at 4:36 AM, Kayashree M wrote:We have a structure which
Dear users,Thank you all for the suggestions.With RegardsKavya--
This message has been scanned for viruses and
dangerous content by
MailScanner, and is
believed to be clean.
Dear users, We have a structure which is a homodimer in the asymmetric unit.PISA predicts most probable assembly as a dimer but thisdimeric assembly is different from what is solved (offcoursewe can generate the symmetry equivalent molecule and get that).My question is - is it necessary that we dep
Dear users, Pardon me for the non-crystallography related question, Can anyone provide some papers regarding the assay ofenzymes from hypertherphilic /thermophilic organisms? ie,assays at high temperatures..Thanking youWith RegardsM. Kavyashree --
This message has been scanned for viruses and
dang
