[ccp4bb] Dissolving deoxycholic acid

Dear All, Initially I planned to wash Inclusion bodies using 1% (w/v) sodium salt of deoxycholic acid (Na-Deoxycholate). In our lab, we have Deoxycholic acid (Not Na-salt). Any inputs how to get it solubilized to reach 1% (w/v)? Thanks Kris

Re: [ccp4bb] problems with cleaving protein

Dear Vivoli, Thanks for your kind inputs. The case of Proline is already taken care of during cloning. I should have pointed this, but thanks that you did mentioned it. Thanks again for inputs. With best regards Rajesh On Sun, Jul 21, 2013 at 12:34 PM, K Singh wrote: > Dear All, >

[ccp4bb] problems with cleaving protein

Dear All, I have expressed fusion protein that is expressed in the following format Fusion partner - Enterokinase cleavage site - Protein of interest. somehow enterokinase is not doing its job though following manufacturers recommendations. any inputs like what protocol worked. Many thanks for y

Re: [ccp4bb] EF motif

> Look at the book Introduction to Protein Structure from Branden & Tooze. > They have great pictures and a very good introduction to fold names. > > Kind regards, > Berta Martins > > > > On Jan 22, 2013, at 2:09 PM, K Singh wrote: > > Dear All, > While goi

[ccp4bb] postdoctoral position in structural neurobiology at Yale University

independently but also be willing to work as part of a team. For more information, please contact me via e-mail. Applicants should send a CV, along with a summary of previous research experience and interests, and arrange to have 3 reference letters sent to Satinder K. Singh via e-mail at

[ccp4bb] postdoctoral position in structural neurobiology

via e-mail. Applicants should send a CV, along with a summary of previous research experience and interests, and arrange to have 3 reference letters sent to Satinder K. Singh via e-mail at satinder.k.si...@yale.edu Additional contact information is given below: Satinder K. Singh, Ph.D

Re: [ccp4bb] Akta Prime / FPLC Options / Off Topic

Dear Mike Even Knauer (though known for HPLCs) are up with the Bioline chromatography solutions. And cheaper than GE. With best regards Kris On Thu, Oct 13, 2011 at 6:48 AM, Paul Smith wrote: > Michael, > Unfortunately, I actually don't know who serves these machines apart from > GE. > Because yo

[ccp4bb] ion exchange: protein peak also in water!!

Dear All, Apologies for bit off-topic. I am in process of purifying a protein by ion-exchange (cation) using 0.5-3M NaCl gradient. And I get a sharp peak which is western positive. Following elution, I wash the column >10 column volume with 3M NaCl. and now when I wash the column with water, I ge

[ccp4bb] Silver staining Coomassie stained gels

Dear All, Can anyone suggest me a protocol for silver-staining the PAGE that is already stained with Coomassie. Kris

[ccp4bb] postdoctoral position at Yale University

itter transporter structure. Channels 2, 380-389 Applicants should send a CV along with a one-page summary of previous research experience and interests and arrange to have 3 reference letters sent to satinder.k.si...@yale.edu - Satinder K. Singh,

[ccp4bb] initial model built in Fo map not refining

Hello, I have a SAD data set to 2.9A and a native data set on the same protein to 2.3A. I have built 70% of the model (polyalanine) into the SAD experimental map that has been subjected to 2-fold NCS averaging and phase-extended to 2.3 A. From the maps, the model looks like it fits the density