Hi all,
There is an assistant prof position currently being advertised at the
fantastic University College Dublin (UCD) that I think will be of interest
to members of the ccp4 community.
“Assistant Professor in Macromolecular Biophysical Chemistry / Chemical
Biophysics, School of Chemistry, Schoo
I have always performed TEV cleavage after eluting my protein from the
Ni-NTA column (TEV cleavage performed during overnight dialysis in
imidazole free buffer as imidazole inhibits TEV activity at high
concentrations). I use TCEP as reducing agent and have never included EDTA,
by this method I've
and heating the sample to accelerate the
oxidation process.
King Regards
Jonathan Bailey (PhD student)
Professor Martin Caffrey Lab MS&FB group Trinity College Dublin
