[ccp4bb] Demonstration for 2nd graders?

Hi everyone, Slightly off topic here but I got myself volunteered by my 2nd grade son to do a show and tell at his class. I have the rock candy experiment ready with some background info on what I do. Can anyone direct me to some resources or your personal demo's that you have done? Thanks a bun

[ccp4bb] fact check about transcription factor binding

I have a structure of a transcription factor in which the recognition helix from two different protomers bind to the same (stretched out) major groove. I was curious whether anyone have seen this kind of motif before - two anti-parallel helices in same major groove. I'm searching the pdb/google bu

Re: [ccp4bb] Yeast membrane protein extraction

Hi Theresa, I totally agree with the bead beater idea. Good reference for recipe (pg.699-701): Overexpression and Purification of Integral Membrane Proteins in Yeast Franklin A. Hays,*,1 Zygy Roe-Zurz,† and Robert M. Stroud -John On Wed, Oct 8, 2014 at 7:25 AM, Theresa Hsu wrote: > Dear

Re: [ccp4bb] Trouble cleaving SUMO tag off of membrane protein

orse in OG), and my solution was to add more Ulp1 and it worked beautifully and I've gotten nice crystals of my protein. -john lee On Sun, Feb 16, 2014 at 9:58 AM, Raji Edayathumangalam wrote: > Hi Everyone, > > After several attempts to cleave the SUMO tag off my membrane

Re: [ccp4bb] Membrane Protein expression and purification

Hi Krish, Seconding Tim's comments, you may need to switch construct or expression host. I've seen many times when a membrane protein seems to be in the yeast membrane, but seems unextractable or most likely, improperly folded. Since you're already working with yeast, I would recommend trying S.ce

Re: [ccp4bb] Membrane Protein Optimisation

Hi Rhys, The bOG concentration of 1.0% is quite acceptable and if your protein is stable and happy in OG, then you're in luck. At 30% PEG 600, I'm not surprised you're seeing the phase separation or detergent 'blob's for lack of a better term. I believe you have the latter, which many of us workin

Re: [ccp4bb] hydrohyapatite column

Hi Sebastiano, Just to add to Dima's response, I've used ceramic hydroxyapatite column and got great results separating 2 very large membrane proteins complexes that eluted together in DEAE/Q and ran in similar size range in SEC. One eluted in 100-200mM KPi and the the other in 300mM+ KPi ~pH 7.4.