Hi Theresa, I totally agree with the bead beater idea.
Good reference for recipe (pg.699-701): Overexpression and Purification of Integral Membrane Proteins in Yeast Franklin A. Hays,*,1 Zygy Roe-Zurz,† and Robert M. Stroud -John On Wed, Oct 8, 2014 at 7:25 AM, Theresa Hsu <theresah...@live.com> wrote: > Dear all > > Would you have a good protocol for membrane protein extract from S. > cerevisiae? We don't have French press in our lab. Is the Pierce Mem-PER > reagent kit useful for proteins (10-12 transmembrane helices) that will be > use for crystallization? > > Thank you. > > Theresa >
