[ccp4bb] PhD positions in structural biology at the Astbury Centre, University of Leeds

Dear CCP4BB members, We invite applications for two PhD positions in structural cell biology based partly or entirely at the Astbury Centre for Structural Molecular Biology, University of Leeds: - *The structure and function of retinal photoreceptor connecting cilium proteins (**Closing date:

[ccp4bb] tenure-track academic fellowships in structural molecular biology at the University of Leeds

Hello everyone, Please see the job advert below regarding tenure-track academic fellowships in structural molecular biology at the University of Leeds. Cheers, Joe Dear All, A number of tenure-track academic fellowships in Structural Molecular Biology are now available at the University of

[ccp4bb] Post-doctoral position at the Astbury Centre, Leeds, UK

Dear All, A post-doctoral position is available immediately in the laboratory of Dr Joe Cockburn, at the Astbury Centre, University of Leeds, UK to perform structure-function studies on ciliary transition zone proteins. The transition zone consists of several large multi-protein complexes at the

[ccp4bb] Post-doctoral position in Structural Biology of Cilia, Astbury Centre, Leeds, UK

Dear All, A post-doctoral position is available immediately in the laboratory of Dr Joe Cockburn, at the Astbury Centre, University of Leeds, UK to perform structure-function studies on ciliary proteins. The position is funded by The Wellcome Trust and is available immediately for a period of

[ccp4bb] DMMULTI question

Dear BB, We are trying to solve the structure of a complex between two proteins. We have two crystal forms of the complex, and a partial MR solution in each. We now want to average the density between these two forms to improve the maps, using DMMULTI. However, there are a couple of things I don't

Re: [ccp4bb] FW: pdb-l: Retraction of 12 Structures

I agree with Randy Read though - it's a mistake to get too carried away with the foul-play aspect of this. There were clearly very serious problems with those structures anyway. What it shows is that you can desposit just about anything in the PDB, which is quite worrying when you consider how much

Re: [ccp4bb] negative density peaks where there is no model.

Hi Andrew, If there is no *protein* model built into this region of the map, then it will be modelled by the bulk solvent correction - therefore, the negative peaks are telling you that the mean electron density there is lower than in the bulk solvent. Probably. Joe > Hello everyone I have a ques

Re: [ccp4bb] Scalepack error model?

Dear Richard, I *think* it works like this, don't know if it's detailed or rigourous enough for you! If I(l,h,i) is the intensity of the ith observation of reflection h on frame l, with error sig(l,h,i), and S(l) is the (inverse) scale factor to be applied to frame l, then the error in the scaled

Re: [ccp4bb] Scalepack error model?

Dear Richard, I *think* it works like this, don't know if it's detailed or rigourous enough for you! If I(l,h,i) is the intensity of the ith observation of reflection h on frame l, with error sig(l,h,i), and S(l) is the (inverse) scale factor to be applied to frame l, then the error in the scaled

Re: [ccp4bb] Protein Color

Hi Matt, I sometimes see a similar thing with my proteins, which definitely don't possess metal co-factors or prosthetic groups. I found that gel filtration got rid of it - the browny-yellow stuff came out in the void fraction so I figured it was aggregated protein. I think it was aggregation via t

Re: [ccp4bb] Question re: ice rings in diffraction data

Dear Mona, Yes. You can get Denzo to reject reflections on or close to ice rings, or any other features with highly irregular background variations, by increasing the value of the REJECT keyword (see p. 40 of the HKL2000 manual by D. Gerwith, http://www.hkl-xray.com/hkl_web1/hkl/manual_online.pdf).