Re: [ccp4bb] zinc with HEPES

2012-05-11 Thread James Kiefer
s. I tried both ZnCl2 and Zn > acetate the effect is same. > I dont know why this Zn in not compatible with HEPES. > Could you please tell me why is this? > I appreciate your help. > > Thanks > Rajesh > -- * * * James Kiefer, Ph.D. * Structural Biology Genentech, Inc. 1 DN

Re: [ccp4bb] Ligand fitting into density

2012-04-11 Thread James Kiefer
ge.Any > unauthorized review, use, disclosure, dissemination, forwarding,printing or > copying of this email or any action taken in reliance on this e-mail is > strictly prohibited and may be unlawful. > > Visit us at http://www.aurigene.com > -- * * * James Kiefer, Ph.D. * Structural Biology Genentech, Inc. 1 DNA Way, Mailstop 27 South San Francisco, CA 94080-4990

Re: [ccp4bb] zinc finger

2012-04-03 Thread James Kiefer
indicated that you had no diffraction yet. Does that mean you have crystals??? If so, what percent of your produced protein is the putative zinc finger region? If it is high, and you already have crystals, then you may be searching for a problem that does not exist. -- James Kiefer, Ph.D

Re: [ccp4bb] Requested: Three-Day Data Fabrication Workshop

2012-04-02 Thread James Kiefer
t; in > the recent curmudgeonry postings. > > > *** > Jacob Pearson Keller > Northwestern University > Medical Scientist Training Program > email: j-kell...@northwestern.edu > *** -- James Kiefer, Ph.D. Structural Biology Genentech, Inc. 1 DNA Way,  Mailstop 27 South San Francisco, CA 94080-4990

[ccp4bb] OPENING: Genentech, Research Associate/Senior Research Associate in Purification and Crystallization

2012-02-22 Thread James Kiefer
candidates with a track record of success in challenging systems such as membrane proteins, multi-domain proteins, or protein:protein (or protein:nucleic acid) complexes. Please encourage qualified candidates to apply to the Genentech website (details below). All the best, Jim * James Kiefer, Ph.D