Dear all,
I have a structure solved by molecular replacement. The C-terminus at
the end of the chain shows as CO, not COOH. How do I make it into COOH
in COOT? I tried to place an O in the relevant position, but seems not
working this way.
Thanks,
Hubing
ll DNA and a much bigger
> protein molecule or if the search models and the molecules have identical
> sequences.
>
> To solve this structure you probably have to do Se-labeled protein and SAD
> etc. or collect anomalous from metal ions if present.
>
> Cheers.
>
> Ray B
space group is P21 and not P2
> or even something else? Did you test other possible space groups? Choosing
> the wrong space group could exactly lead to the results you observe.
>
> Best,
> Herman
>
>
> --
> *From:* CCP4 bulletin board [mailt
< 5, have I solved it ? No.
>
> Hence with a TFZ score of 3.8 you do not have a solution using Phaser.
>
> Fred.
>
> Hubing Lou wrote:
>
>> Dear all,
>>
>> I am stuck in a molecular replacement case and looking for advices.
>> I have been working on a
>
>
>
> Tel. 01235 778057
>
> Mob. 07920 138148
>
>
>
> *From:* CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] *On Behalf Of
> *Hubing
> Lou
> *Sent:* 24 November 2010 14:09
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* [ccp4bb] unusual diffraction spot
d Light Source
>
> Harwell Science and Innovation Campus
>
> Chilton
>
> OX11 0DE
>
> UK
>
>
>
> Tel. 01235 778057
>
> Mob. 07920 138148
>
>
>
> *From:* CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] *On Behalf Of
> *Hubing
> Lou
> *Se
You didn't mention which detergent in the conditions. From the picture it looks
like crystals of detergent.
--
Hubing Lou
Centre for Biomolecular Sciences
University of St Andrews
North Haugh
St Andrews
Fife
KY16 9ST
Scotland
Quoting Ngo Duc Tri <[EMAIL PROTECTED]>:
> Dear c
First of all, thanks very much for those who replied to my question.
For those who do not follow this thread, I was asking a cryo question about
membrane protein crystals grown at 4C with 0.05-0.2M AS (ammonium sulfate) and
15-26%PEG400.
Some of you suggested directly freeze the crystal in LN fro
Dear everybody,
I have crystallized a membrane protein at cold room temperature (4°C). The
protein was purified in 20mM Tris, pH8 with 1% bOG. The reservoir solution
contains 0.1M HEPES pH7.5, 0.05-0.2M (NH4)2SO4 and 15%-26% PEG400.
The cryoprotectant was made in such a way that all the other ing
