Hi, The "ears" extended to other images but not all of them, some images do not show any signs like this. Thanks for replying, Liz.
Best, Hubing On Wed, Nov 24, 2010 at 10:26 PM, <elizabeth.d...@diamond.ac.uk> wrote: > I think there may be two effects going on here: > > > > I think the “ears” on the round spots which also feature on the more rod > shaped spots if you look closely could be related to a misalignment of the > beamline optics. > > > > I think the change in spot shape from round to rod shaped is due to the > crystal quality. > > > > Do the “ears” only feature on this image of this crystal or do they appear > on other images? If the ear effect is a one off then that would tend to > suggest it isn’t a beamline optic effect. > > > > Liz > > > > Dr. Liz Duke > > Principal Beamline Scientist > > Diamond Light Source > > Harwell Science and Innovation Campus > > Chilton > > OX11 0DE > > UK > > > > Tel. 01235 778057 > > Mob. 07920 138148 > > > > *From:* CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] *On Behalf Of > *Hubing > Lou > *Sent:* 24 November 2010 14:09 > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] unusual diffraction spots > > > > Dear CCP4BBer, > > I recently collected a dataset at synchrotron. The diffraction was quite > anisotropic with one direction to 2.1Angstrom while the other is 3.0Ang. > What unusual is in the diffraction image (see the attached file), clearly at > low resolution there were some spots with tails ("two ears") and at the high > resolution shell the spots turned to be rod-shaped. Please, can anyone > explain how this could be? Is this related to the anisotropy? The protein > was N-terminal his6-tagged, we are currently preparing new samples with > the His-tag removed. But any other suggestions are also very welcomed. > > Regards, > > Hubing > > > > -- > > This e-mail and any attachments may contain confidential, copyright and or > privileged material, and are for the use of the intended addressee only. If > you are not the intended addressee or an authorised recipient of the > addressee please notify us of receipt by returning the e-mail and do not > use, copy, retain, distribute or disclose the information in or attached to > the e-mail. > Any opinions expressed within this e-mail are those of the individual and > not necessarily of Diamond Light Source Ltd. > Diamond Light Source Ltd. cannot guarantee that this e-mail or any > attachments are free from viruses and we cannot accept liability for any > damage which you may sustain as a result of software viruses which may be > transmitted in or with the message. > Diamond Light Source Limited (company no. 4375679). Registered in England > and Wales with its registered office at Diamond House, Harwell Science and > Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom > > >
