Re: [ccp4bb] Pymol Map range

Thanks Paul, that has worked just fine. Cheers, Sam From: CCP4 bulletin board On Behalf Of Paul Emsley Sent: 26 February 2021 16:12 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Pymol Map range On 26/02/2021 16:07, Horrell, Sam (DLSLtd,RAL,LSCI) wrote: Hello BB, I'm trying to ma

Re: [ccp4bb] File System Problems

Hi Stuart, That has cracked It thanks! I can open files again. Much appreciated, Sam From: Stuart McNicholas Sent: 01 October 2020 13:34 To: Horrell, Sam (DLSLtd,RAL,LSCI) Cc: CCP4BB@jiscmail.ac.uk Subject: Re: [ccp4bb] File System Problems Dear Sam, We have heard reports that running as

[ccp4bb] File System Problems

Hello CCP4bb, I'm having a serious problem with my CCP4i2 installation on my windows 10 computer, whenever I try and upload a file via the GUI it fails to open the file system and stops responding, then it just hangs until I kill the program. I've uninstalled and reinstalled CCP4 and rolled bac

Re: [ccp4bb] Coot CCP4i2 extensions

HI Stuart, I’m not sure what has changed but the extension menu is back and working now. I guess it sorted itself out somewhere between opening and closing ccp4i2 and restarting my computer a few times. Cheers, Sam From: Stuart McNicholas Sent: 04 June 2020 14:09 To: Horrell, Sam (DLSLtd

Re: [ccp4bb] Coot CCP4i2 extensions

:46 To: Horrell, Sam (DLSLtd,RAL,LSCI) Subject: Re: [ccp4bb] Coot CCP4i2 extensions Coot should write a log somewhere - not sure where it is - and that will record problems reading startup scripts. If you can find it it might be useful. Paul. On 04/06/2020 11:43, Horrell, Sam (DLSLtd

Re: [ccp4bb] Coot CCP4i2 extensions

path to the coot executable in the preferences is blank but if there was a problem I don’t think coot would have loaded at all from the interface. Cheers, Sam From: Stuart McNicholas Sent: 04 June 2020 11:38 To: Horrell, Sam (DLSLtd,RAL,LSCI) Cc: CCP4BB@jiscmail.ac.uk Subject: Re: [ccp4bb] Coot

[ccp4bb] Coot CCP4i2 extensions

Hello CCP4bb, I just installed the latest update and it seems to have a problem with Coot's CCP4i2 extensions. The dropdown menu no longer appears when clicked so it's impossible to save to the i2 gui. I've rolled back the update but the bug is still present. Cheers, Sam -- This e-mail and

Re: [ccp4bb] CCP4i2 Text size

:04 To: Horrell, Sam (DLSLtd,RAL,LSCI) Cc: CCP4BB@jiscmail.ac.uk Subject: Re: [ccp4bb] CCP4i2 Text size Hi Sam, On 2 Apr 2020, at 11:32, Horrell, Sam (DLSLtd,RAL,LSCI) mailto:sam.horr...@diamond.ac.uk>> wrote: If anyone has any other suggestions it would be greatly appreciated. What set

Re: [ccp4bb] teaching question: graphics for chromebook?

Being on the ccp4bb I feel I should ask if ccp4mg isn't suitable for your purposes? Sam From: "Phoebe A. Rice" To: "ccp4bb" Sent: Wednesday, 23 May, 2018 22:01:16 Subject: [ccp4bb] teaching question: graphics for chromebook? One for fellow educators: For years, I’ve given students

[ccp4bb] CCP4i2 Error in wrapper

Hello CCP4bb, I'm having some trouble with my installation of CCP4i2. I keep getting a persistent error about the "Wrapper" in various programs and am not sure what I need to do to fix it. I have tried reinstalling via the package manager (64b linux) and still get the error. So far it has happ

Re: [ccp4bb] Manual His-Tag Purification

This should give you everything you need to know about the column http://www.sigmaaldrich.com/technical-documents/protocols/biology/roche/complete-his-tag-purification-column.html I'd definitely say load all of your lysate, possibly run it through twice if you really want to. If you're worried

Re: [ccp4bb] off topic pymol

Hi Almudena, Have a look in your PDB file in the second to last colum, if it looks something like the example below then delete the As from the fine and it should connect up. I've had this before, not sure where it came from but removing it fixed the problem. ATOM371 CA PRO A 48 -11

Re: [ccp4bb] fitting peptide with non-standard and standard amino acids

Hi Emmanuel, I've never tried something quite as complex as a pseudopeptide but if you can build your lingand in the coot ligand builder coot will generate a cif file for you. Previously I have built my ligand, used real space refinement to get it into position, merged the protein and ligand a

Re: [ccp4bb] Processing files from XSCALE in Aimless

in aimless. Are you certain that this is good for the signal in your data? best, Kay > >Hope this helps, > >Regards, > >Joe > >-Original Message- >From: CCP4 bulletin board on behalf of Horrell, Sam >Sent: Fri 7/26/2013 1:32 PM >To: CCP4BB@JISCMAIL.AC.UK &g

[ccp4bb] Processing files from XSCALE in Aimless

Hi Everyone, I've been trying to process some data using Xia2, XSCALE, Pointless and Aimless. I have a large number of datasets (over 100) so have used Xia2 to produce the XDS_ASCII.HKL files using the 3daii pipeline. I now want to process them manually (with a script) using XSCALE and pointles