[ccp4bb] Deadline extended: Postdoc and PhD positions in mitochondrial molecular & structural biology

Dear all, We have extended the application deadline for two fully-funded open positions, one for a postdoctoral researcher and one for a PhD student, in our research group located at the University Medical Center Göttingen and the Max-Planck-Institute for Multidisciplinary Sciences in Göttingen

[ccp4bb] Open Postdoc and PhD positions in mitochondrial molecular & structural biology

Dear all, We have two fully-funded open positions, one for a postdoctoral researcher and one for a PhD student, in our research group located at the University Medical Center Göttingen and the Max-Planck-Institute for Multidisciplinary Sciences in Göttingen, Germany. Our team studies the struc

[ccp4bb] PhD position in Structural Biology of Mitochondria

Dear all, I have an open PhD student position in my research group, which is joint between the University Medical Center Göttingen and the Max-Planck-Institute for Biophysical Chemistry in Göttingen, Germany. Our team studies the structure and function of molecular machineries involved in gene

Re: [ccp4bb] Coot issue with add residue

Dear all, Thank you for your many suggestions! Indeed, I was not able to fix the issue to my satisfaction. As Paul suspected, set_add_terminal_residue_do_post_refine was already set to 0. The triple-refine also did not improve anything. I also checked for the TER records, which I also suspected

[ccp4bb] Coot issue with add residue

Hi All, Lately I have been having a strange issue with Coot (0.8.9.2-pre on MacOS X). Sometimes when I add residues using “Add residue…”, they are not linked to the previous residue. When I try to real space refine / normalize they behave as if they were part of the chain, but the atoms that sh

Re: [ccp4bb] intensity statistics and twinning

e, when you use twin refinement, your R-factor is bound to drop whether it is twinned or not. In your case Rfree rises, which is actually hinting at your data not being twinned. How many reflections do you have in the Rfree set? Best, Tim On Friday, October 28, 2016 1:59:29 PM CEST Hillen, Hauke

Re: [ccp4bb] intensity statistics and twinning

Dear Kay, Thank you for your answer! I have meanwhile processed my anomalous data also also as P1 and solved the structure with MR-SAD in P1, which works flawlessly and gives the same solution. Compared to P21, I now have 4 instead of 2 molecules in the ASU, which makes sense. I then tried refi

[ccp4bb] AW: [ccp4bb] AW: [ccp4bb] intensity statistics and twinning

Agreed, and this is ongoing work. Yet it is not trivial to get better resolution than this for small (in the world of EM) samples either. Von: Mark van Raaij [mjvanra...@cnb.csic.es] Gesendet: Donnerstag, 27. Oktober 2016 22:08 An: Hillen, Hauke Betreff

[ccp4bb] AW: intensity statistics and twinning

: Donnerstag, 27. Oktober 2016 21:36 An: Hillen, Hauke; CCP4BB@JISCMAIL.AC.UK Betreff: RE: intensity statistics and twinning It would be helpful to know what your current R values are in modelling, and also the merging statistics. It looks like you might have a twinned p1 crystal. JPK From: CCP

[ccp4bb] AW: [ccp4bb] intensity statistics and twinning

, Hauke Von: Mark van Raaij [mjvanra...@cnb.csic.es] Gesendet: Donnerstag, 27. Oktober 2016 21:29 An: Hillen, Hauke Betreff: Re: [ccp4bb] intensity statistics and twinning I'd say intensity statistics at this resolution at not reliable and your crysta

[ccp4bb] intensity statistics and twinning

Dear ccp4 community, I am currently working on some low resolution datasets (around 4.5A). The space group seems to be P21, as suggested by XDS and pointless. I have collected many datasets of these crystals, both native as well as SeMet-labeled. Using MR-SAD I have been able to obtain a clearl