[ccp4bb] Na/K Phosphate

2009-05-14 Thread Geoffrey Kwai Wai Kong
Dear all, Quite a few crystallisation conditions in the screens feature 'sodium/potassium phosphate'. I'm curious to know why such a Na/K mix is there. As the pH is mostly determined by the (H2PO4)- to (HPO4)2- ratio, is there such a need to have both cations? If so, is the Na to K ratio important

Re: [ccp4bb] : Constrain Metal ligand bond distance

2007-01-24 Thread Geoffrey Kwai Wai Kong
Hi, From my rather limited experience ... Is your EXAFS data collected on a crystal or in solution? If the latter, it is worth remembering that the chemical environment in solution and in crystal can be different. Moreover, atomic coordinates and bond distances determined via crystallography a