[ccp4bb] E.coli strain, knock-out for SDH/FR

2017-04-04 Thread Fulvio Saccoccia, Sapienza
Dear ccp4ers, I was wondering if someone does know (and hence could let me know in turn) an E.coli strain which lacks both of the sdh (succinate dehydrogenase) and frd (fumarate reductase) operon, so that bacteria do no longer retain any activity from complex II or similar. I know the que

[ccp4bb] advances in multisubunit membrane protein

2017-02-03 Thread Fulvio Saccoccia, Sapienza
Dear ccp4ers, I was wondering about most recent advances in production and crystallization of complexes of multisubunit membrane proteins, for instance succinate dehydrogenase, pyruvate dehydrogenase complex or even photosystem complex. As far as I know, many large complexes of membrane pr

[ccp4bb] Fwd: [ccp4bb] XDS INP

2015-05-07 Thread Fulvio Saccoccia, Sapienza
Dear Lu you can freely download the tutorial from here: http://strucbio.biologie.uni-konstanz.de/xdswiki/index.php/Main_Page You can check if your detector is supported here: http://xds.mpimf-heidelberg.mpg.de/html_doc/detectors.htm Best Fulvio > Il giorno 07/mag/2015, alle ore 10:33, luzu

Re: [ccp4bb] how to recover my data

2015-03-05 Thread Fulvio Saccoccia, Sapienza
ore 16:18, Harry Powell ha scritto: > Hi Fulvio > > MTZ files are binary, so I don't think they have line-endings in the sense > that text files do. > > On 5 Mar 2015, at 15:09, Fulvio Saccoccia, Sapienza wrote: > >> Sorry, my previous post was not properly att

Re: [ccp4bb] how to recover my data

2015-03-05 Thread Fulvio Saccoccia, Sapienza
Sorry, my previous post was not properly attached; I attached my idea: Dear Smith, It is curious; I am not sure about what I am going to suggest since I never had that problem but the simplest explanation I found is that you have some Line Endings issues. Windows, Mac and Unix all use differe

[ccp4bb] Fwd: [ccp4bb] how to recover my data

2015-03-05 Thread Fulvio Saccoccia, Sapienza
Inizio messaggio inoltrato: > Da: "Fulvio Saccoccia, Sapienza" > Oggetto: Re: [ccp4bb] how to recover my data > Data: 05 marzo 2015 13:48:34 CET > A: Smith Lee > > Dear Smith, >It is curious; I am not sure about what I am going to suggest since I &