[ccp4bb] OASIS new version released

Dear colleagues, We are pleased to announce the release of OASIS version 4.2 and the pipeline IPCAS (Iterative Protein Crystal-structure Automatic Solution) version 1.0, which combines OASIS with other protein crystallographic programs to perform automatic iterative direct-method phasing, density

Re: [ccp4bb] Direct method solution at 1.15A

Dear Yuri, If you have located all the heavy atoms (sulfur and phosphor) correctly, you could try sulfur-SAD phasing using the program OASIS. This program has a record of solving a 1206 residues protein with SAD signals from 22 sulfur atoms scattered under Cu-Ka radiation and a record of solving a

Re: [ccp4bb] how to combine the experimental phase and molecular replacement phase

Hi Jiamu, OASIS may help solving your problem. We have example on solving an originally unknown protein (1086 residue in the AU) at 3.3A resolution with Se-SAD data (redundancy=3.3). The process involved SAD-iteration and MR-iteration with OASIS. If you need help, we can discuss off list. Cheers

Re: [ccp4bb] low res. SAD phasing

Concerning "low resolution SAD phasing" I would like to add some information here: 1. OASIS (version 2000) helped solving the unknown structure 1u9s in phasing the 2.9A SAD data ( http://www.sciencemag.org/content/suppl/2004/09/30/306.5693.104.DC1/Krasilnikov.SOM.pdf ). 2. OASIS-2004 helped solvi

[ccp4bb] OASIS version 4.0

Dear colleagues, We are pleased to announce that OASIS version 4.0 is now available. This version includes an improved SAD-phasing algorithm and a GUI for controlling and monitoring Dual-space iteration processes, which involve phasing, density-modification and model-building programs. More detail

Re: [ccp4bb] how to bring back the missing density for half of the structure

Dear Eric, For your question 2, the following paper provides some examples: Acta Cryst. D63, 793-799 (2007). In one of the examples, there is shown a partial model from Phaser containing ~20% residues in the ASU can be extended to more than 90% of the ASU by the iteration of ARP/wARP-OASIS-DM. D

[ccp4bb]

into the current version of CCP4 suite. For more details please visit our Webpage at http://cryst.iphy.ac.cn <http://cryst.iphy.ac.cn/> . The program is freely available for academic purpose. Fan, Hai-fu Institute of Physics Chinese Academy of Sciences Beijing 100080 P.R. China

Re: [ccp4bb] Solving a structure by MR with a pseudo-translation vector

modulated structures can be found on the Webpage: http://cryst.iphy.ac.cn <http://cryst.iphy.ac.cn/> Hai-fu -- Professor Fan, Hai-fu Institute of Physics Chinese Academy of Sciences Beijing 100080, P.R. China Email: [EMAIL PROTECTED] URL: http://cryst.iphy.ac.cn

Re: [ccp4bb] Commensurate superlattices in protein crystallography?

available yet for this purpose. Regards, Hai-fu Professor Fan, Hai-fu Institute of Physics Chinese Academy of Sciences Beijing 100080, P.R. China E-mail: [EMAIL PROTECTED] URL: http://cryst.iphy.ac.cn <http://cryst.iphy.ac.cn/> _ From: CCP4 bulletin board [