Dear all,
Thanks a lot for good suggestions on this topic. To answer some of the
questions: The apo strucuture has not been solved; the crystals dissolved upon
soaking (my term 'crack' was not accurate); and I have not tried
co-crystallization.
A brief summerization here:
1) Soak/Co-crystalli
,
Dianfan
Dianfan Li, PhD
College of Biochemistry and Immunology
Trinity College Dublin
Dublin, Ireland.
Dear all,
I wish to thank everybody for all the suggestions, pictures and ideas!
Dianfan
Hi,
I only read the topic this morning -
In some cases, I have to wait 30+ hours to see colonies when I transform
BL21 DE3 Star while normally they should appear after 14 hours.
Dianfan
ar...@xtals.org wrote:
Hello
In order to know *exactly* what the reason for poor transformation outcome
