Le Mardi 7 Juillet 2015 14:03 CEST, Almudena Ponce Salvatierra
a écrit:
Hello
Do you have full complementarity of DNA and RNA ?
Does the difference in lenght (17 nt/19 nt) mean that you have a bulge on the
DNA when the duplex is formed ?
In short, what is the Tm of this duplex at these strand
uniform
> > background in the X-ray diffraction patterns.
> >
> > I do not have a copy, but if I remember well it was done with a copper
> > wire, hair and nylon
> > loops came later.
> > Daniel
> >
> >
> >
> > Le 03/11/2014 18:23, DUMAS Philippe (VIE) a
I am looking for the reference of this little paper (in J. Appl. Cryst ?)
describing how to make a loop and fish crystals...
You know, this kind of humble method used by all crystallographers (apart FEL
users).
Thank you in advance
Philippe Dumas
Le Vendredi 14 Mars 2014 13:37 CET, Anita P a écrit:
Anita,
If one of the partners is indeed more or less unfolded before interaction,
then you should see a negative DeltaS upon complex formation.
Practically, I would try first putting the unfolded protein in the cell and the
folded one in t
Dear all,
By some extraordinary chance, could someone provide me information about the
temperature dependence of two E. coli enzymes:
(1) Hydroxyethythiazole kinase: EC 2.7.1.50 (encoded by the gene thiM)
(2) Enzyme participating in the synthesis of 4-amino-5-hydroxymethyl pyrimidine
phosphate
