Dear all, By some extraordinary chance, could someone provide me information about the temperature dependence of two E. coli enzymes: (1) Hydroxyethythiazole kinase: EC 2.7.1.50 (encoded by the gene thiM) (2) Enzyme participating in the synthesis of 4-amino-5-hydroxymethyl pyrimidine phosphate from 5-aminoimidazole ribotide (encoded by the gene thiC)
Something like kCat(T), Km(T) would be magnificent. And if I am very, very lucky: more generally, any information/reference on the temperature dependence of other enzyme(s) involved in thimamine pyrophosphate biosynthesis would be much welcome. Many thanks in advance. Philippe Dumas Biophysics & Structural biology team IBMC-CNRS, 15 rue René Descartes Strasbourg, France +33 (0)388 41 70 02
