[ccp4bb] xorg.conf set-up for Ubuntu with NVIDIA 980 xgl and Crystal Eyes

(replaced batteries as well), - the monitor splits the image as expected, but: - the emitter is not flickering and the crystal eyes do not become active. Anybody has solved this problem before, please? I couldn't find it in previous threads... Any help would be very much appreciated. Claudi

[ccp4bb] Off-topic - Domain termini for construct design

termini of a protein of interest when it is not important to keep them "true" (=native) in order to optimise expression levels. Is there any good reference about this, please? Personal experiences are welcome. Many thanks, Claudia Claudia Scotti Dipartimento di Me

[ccp4bb] Freezing under oil - Thanks and summary

w? If its sodium acetate and ammonium sulphate I would try adding sodium malonate as a cryo protectant (with 2-2.3M ammonium sulphate adding 0.6-0.8M Na malonate should be sufficient) or increasing the ammonium sulphate concentration. Claudia Scotti Dipartimento di Medicina Sperime

[ccp4bb] Freezing under oil

iences and suggestions are welcome. Claudia Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0

Re: [ccp4bb] Warren DeLano

Claudia Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0039 0382 303673 Date: Thu, 5 Nov 2009 10:54:13 -0800 From: brun...@stanford.edu Subject: [ccp4bb] Warren De

[ccp4bb] Test of cryoconditions without X-rays or cryostream

under the microscope (or by eye) or is this still risky? Thanks, Claudia Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0

[ccp4bb] Linux flavour and hard disks

Thanks a lot, Claudia Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0039 0382 303673 _ More than mess

[ccp4bb] Off-topic: modelling of a transmembrane domain

MMSTr-Rosetta models it as a beta-sheet. Please, would anyone have any suggestions on how to proceed or might please someone indicate a more appropriate forum/list for modelling problems? Many many thanks, Claudia Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Pato

[ccp4bb] Contact distances and medium resolution structures

Dear List, Please, is there anybody who could suggest me a good review or some references concerning the relationship between the exerimental uncertainity of distances in medium resolution crystal structures (specifically 2.5-2.9 A) and appropriate cut-off distances for hydrogen bonds, hydrop

[ccp4bb] Morphing with mutations

sual effect? Does LSQMAN, for example, sort this problem out? It is not clear to me reading the manual. Many many thanks, Claudia Claudia Scotti> Dipartimento di Medicina Sperimentale> Sezione di Patologia Generale> Universita' di Pavia> Piazza Botta, 10> 27100 Pavia> It

[ccp4bb] How to replace 2.45M K/Na phosphate buffer - SUMMARY

ic:dibasic phosphate ratio in the soak buffer once the crystals have grown to try and stabilize your crystals at a lower phosphate concentration or in the presence of the cryoprotectant. Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Pi

[ccp4bb] How to replace 2.45M K/Na phosphate buffer - 2

Sorry: forgotten the pH: the crystals grow between pH 6.5 and 6.7. Claudia Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di Patologia Generale Universita' di Pavia Piazza Botta, 10 27100 Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0039 0382 3

[ccp4bb] How to replace 2.45M K/Na phosphate buffer

rations it is acting as a buffer but also as a precipitant and I was wondering if anybody has experienced nice buffer-salt combinations that proved to be useful starting from a similar condition. Thanks a lot, Claudia Claudia Scotti Dipartimento di Medicina Sperimentale