On 8/13/14 2:29 PM, Theresa Hsu wrote:
Dear all
One of my human membrane proteins have been described to interact with
additional subunits for its activity. To obtain functional form in yeast
(Saccharomyces), I can think of two approach of either cloning all the subunits
under one promoter or
On Friday 18 January 2008 11:18:45 am Mischa Machius wrote:
> OK, that brings us back to a more substantial question: is any of
> these R values actually suitable to judge the quality of a given
> dataset? Instead of introducing novel R factors, one could also simply
> ignore them altogether,
On Friday 18 January 2008 09:30:06 am Ethan A Merritt wrote:
>
> Rmerge is an average over replicate measurements of the intensity for
> identical [hkl]. Rsym is an average over the measurements for all symmetry
> equivalent reflections.
>
> In the presence of anomalous scattering, Rsym will be hig
On Friday 21 September 2007 09:03:56 am Sue Roberts wrote:
> Yes, and I just got a pdb file back from the pdb that they flagged
> with over forty bad residues.
>
> The Molprobity plot from the RCSB validation server shows zero
> residues in disallowed regions. ( I got lucky, I didn't "fix" any bad
On Friday 04 May 2007 01:10 am, Ingo P. Korndoerfer wrote:
> but i think i would be willing to put up with having to add another 10
> lines to my scripts to process mmcif, if
> that allows me to take home all the other advantages.
>
> the biggest problem at this point is, as far as i know (and thi
