[ccp4bb] RES: [ccp4bb] Choosing test set for twin refinement, with multiple operators

Dear all, thank you for the valuable inputs. I realize that one important piece of information that must be added concerns the relative orientation of the domain for which the electron density becomes poor upon merging data towards higher symmetry, with respect to the rest of the protein. We h

[ccp4bb] Choosing test set for twin refinement, with multiple operators

Dear all, We are currently refining a low resolution model (3.9 A max), obtained by MR. Dataset was collected from a single crystal with one long cell axis (~620 A) and high solvent content (74%). Best refinement results (by far!) are obtained in Refmac, by imposing P21 sg (20 multidomain monome

[ccp4bb] Postdoc Position on Structural Biology of Membrane Proteins in Brazil

Dear colleagues, I intend to implement work on structural biology (by X-ray crystallography) of membrane proteins in my lab, more specifically on the subset of inner mitochondrial membrane carriers. For this, I would like to take this opportunity and invite anybody who is prospecting a postdoc

[ccp4bb] Summary: Identity of a Bacterial lipid

hor%5D&cauthor=true&cauthor_uid=11418499>, Svatos A<http://www.ncbi.nlm.nih.gov/pubmed?term=Svatos%20A%5BAuthor%5D&cauthor=true&cauthor_uid=11418499>. Chem Senses.<http://www.ncbi.nlm.nih.gov/pubmed/11418499?dopt=Abstract> 2001 Jun;26(5):529-31. Uma. -- Uma Katre Columbu

[ccp4bb] RES: [ccp4bb] Identity of a Bacterial lipid

Rd., Rm. 513 Michigan State University East Lansing, MI 48824-1319 Office: (517) 355-9724 Lab: (517) 353-9125 FAX: (517) 353-9334Email: rmgarav...@gmail.com<mailto:garav...@gmail.com> On Oct 3, 2013, at

[ccp4bb] Off-topic: heterologous co-expression in yeast

Hi, We have been thinking of implementing recombinant protein expression in S. cerevisiae in our lab, and I would like to ask if there is a choice of vector designed for co-expression of any two intracellular binding partners, similarly to the pETduet-1 vector for E. coli. If you feel this is no

Re: [ccp4bb] Desalting columns

Hi, We have a case where a 260 KDa protein crystallized at 3 mg/ml, that was the highest concentration we could achieve, without playing with the protein buffer. Protein-to-well-solution ratio was 3:1. When screening, we tested all ratios between 4:1 and 1:1. Crystals only appeared at 3:1 ratio.

[ccp4bb] Why 0.1% bandwidth?

Dear ccp4bb, I sometimes find the flux of x-ray sources reported in units of "photons/s/0.1% bandwidth" instead of simply "photons/s". Where does the "1/0.1% bandwidth" unit come from? I have also seen other percentages like 0.01% bw or 0.02% bw... Is it simply defining some degree of acceptanc