Re: [ccp4bb] Off topic ; how to remove fluorescein from superdex increase

Have you tried Guanidinium chloride? Jürgen > On Jul 3, 2024, at 1:22 PM, Daniele de Sanctis wrote: > > Hi Flavio > > I’d suggest to contact Regenfix > https://www.regenfix.eu/ > > Usually they give you back a column better than the original one completed > with an experimental calibration

Re: [ccp4bb] Off topic ; how to remove fluorescein from superdex increase

Hi Flavio I’d suggest to contact Regenfix https://www.regenfix.eu/ Usually they give you back a column better than the original one completed with an experimental calibration curve. Cheers Daniele On Wed 3 Jul 2024 at 18:56, Nicholas Clark < b2b1c7e93c2d-dmarc-requ...@jiscmail.ac.uk> wro

Re: [ccp4bb] Off topic ; how to remove fluorescein from superdex increase

Hi Flavio, I’d recommend reaching out to Cytiva directly ( scientificsupport...@cytiva.com). We had an issue with oxidized metal on our S200 and they were able to provide useful suggestions (plus side is, you know what they suggest won’t ruin the resin). Best, Nick Clark Nicholas D. Clark, PhD

Re: [ccp4bb] Tools for identifying possible contaminants

I think this may do the contaminant search you are looking for. ContaMiner (kaust.edu.sa) Best, Mike On Wed, Jul 3, 2024 at 12:04 PM Kyle Gregory < 3632

[ccp4bb] PDRA position available for structure validation and MR software development at the University of Liverpool

[with apologies if you get this more than once] Dear all A 3-year position is available to develop software for structure validation using Deep Learning-derived predicted residue contacts and distances, and for optimal Molecular Replacement using the results of the latest RNA structure predict

[ccp4bb] Tools for identifying possible contaminants

Dear all, We have a unit cell that is too small for our expected protein and suspect we have crystalised a contaminant. Does anyone have any recommendations on which tools we could use to identify the possible contaminant? I've tried SIMAD on ccp4cloud and it doesn't suggest anything reasonabl

Re: [ccp4bb] ATP analogues

Dear Reza, the stability of these complexes is variable and depends on many factors including your protein, the pH of the buffer etc. I would recommend aluminium fluoride as the most stable (below pH8) as it is present in solution and has a the highest charge to size ratio so binds tightly in

[ccp4bb] ATP analogues

Hi, Sorry for the non crystallography question. We need to make some ATP analogues (ADP BeFx, ADP MgF3, and ADP AlFx). I have attained protocols from manuscripts for making these; however, am concerned that something important in the protocol may be lacking. Also, how stable are these samples?

[ccp4bb] Beamline Scientist and Research Engineer at the MAX IV MX group

Dear all, We have two positions open for applications at the Macromolecular Crystallography group at MAX IV. The group is responsible for the two beamlines BioMAX and MicroMAX and the fragment screening facility FragMAX. * Beamline Scientist: You will contribute to and drive inhouse projects f

[ccp4bb] Instruct-ERIC Structure Meets Function Webinar 33 - Fragment-Screen

Dear All, The 33rd edition of the Instruct-ERIC Structure Meets Function webinar is taking place 16 July, featuring talks from the Fragment-Screen project. Register for the webinar here