Hi Flavio

I’d suggest to contact Regenfix
https://www.regenfix.eu/

Usually they give you back a column better than the original one completed
with an experimental calibration curve.

Cheers

Daniele



On Wed 3 Jul 2024 at 18:56, Nicholas Clark <
0000b2b1c7e93c2d-dmarc-requ...@jiscmail.ac.uk> wrote:

> Hi Flavio,
>
> I’d recommend reaching out to Cytiva directly (
> scientificsupport...@cytiva.com). We had an issue with oxidized metal on
> our S200 and they were able to provide useful suggestions (plus side is,
> you know what they suggest won’t ruin the resin).
>
> Best,
>
> Nick Clark
>
> Nicholas D. Clark, PhD (He/Him)
> University at Buffalo
> Department of Structural Biology
> Jacob's School of Medicine & Biomedical Sciences
> 955 Main Street
> <https://www.google.com/maps/search/955+Main+Street+Buffalo,+NY+14203?entry=gmail&source=g>
> Buffalo, NY 14203
> <https://www.google.com/maps/search/955+Main+Street+Buffalo,+NY+14203?entry=gmail&source=g>
>
>
> On Wed, Jul 3, 2024 at 12:01 PM Flavio Di Pisa <dipi...@gmail.com> wrote:
>
>> Dear community,
>> do you have any advice on how to remove the fluorescein from a superdex
>> 200 increase?
>> Thank you
>> Flavio.
>>
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