Is there a specific operation instruction for microseed matrix screening (MMS),
according to the hampton research manual?
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Hi Mark,
Gentauer may still sell those…
https://maxanim.com/strains/m15-prep4-escherichia-coli-strains/
Cheers,
Jeroen
__
Dr. math. et dis. nat. Jeroen R. Mesters
Biological Safety Officer (BBS)
Deputy, Lecturer, Program Coordinator Infection Biology
Visiting Professorship in Biophysics South B
In fall of 2024, electron density map coefficients will be available in
the public PDB archive for all X-ray structures. These map coefficients
will be the same as used in wwPDB Validation Reports.
The new map coefficients files will replace the electron density maps
and combined map coefficie
We got an aliquot of SG13009 once from
https://labs.utsouthwestern.edu/jiang-youxing-lab back when I was a postdoc at
UT Southwestern. I don't have any now.
Doeke
=
Doeke Hekstra
Assistant Professor of Molecular & Cellular Biology, and of Applied Physics
(SEAS),
Director of Undergraduate
Dear All.
we are looking for E. coli strain M15 or SG13009 to use for expressing a
protein from the vector pQE30. Are these strains still availabe commercially?
If not, we'd be open for another way to obtain an aliquot.
Wbw,
Mark
Mark van Raaij
Dpto de Estructura de Macromoleculas, lab 20B
Ce
Hi All,
The deadline for applications is rapidly approaching – please apply by 10th
June.
Dave
-
Hi All,
The John Innes Centre in Norwich (UK) will be hosting this year’s CCP4-BCA
Protein Crystallography Summer School.
Please follow this link for more details and to appl
Dear Lionel,
If you want a programme that takes a PDB file and automatically generates a
topology diagram, then Pro-origami may be the software you need
(https://sites.google.com/site/alexdstivala/home/pro-origami) - it generates
prettier diagrams than PDBsum, and the diagrams are output as .sv
Dear Marco,
Please don't you mind to export your refmac5 job and send it to me so I
can have a look? I will keep it confidential. In the following coot job,
do you open the structure model in PDB or mmCIF format?
You can also export a PDB or a mmCIF file from the refmac5 job -
similarly as a
Your pictures 1 3 & 4 don't seem to show multiple occupancy? Maybe the
difference map 2 is saying - too much complexity ??
Eleanor
On Thu, 30 May 2024 at 23:29, Michael Colaneri
wrote:
> Dear All,
> We have alternate conformations for a number of residues in a structure
> and one is a Val that I
