Dear all,
Please remember to join us tomorrow for our next NIH sponsored Joint CryoEM
Service Centers Webinar: "Building Bridges: Selenocysteine Synthase as a Model
for Efficiently Managing Interactions between Researchers and National
Facilities" by Dr. Vitor Hugo Balasco Serrão at 12 PM East
There's nothing wrong with translational NCS. It is what it is ;-0
Best wishes, Jon Cooper. jon.b.coo...@protonmail.com
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Original Message
On 29 May 2024, 15:49, Kay Diederichs wrote:
> Hi Catherine, I think you meant to write "giving high R-factors"
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Hi Catherine,
I think you meant to write "giving high R-factors".
To add to what Esko and Eleanor wrote: There is nothing wrong with your data or
spacegroup or refinement; it is just that the tNCS produces two sets of
reflections: half or your reflections are statistically "normal" (as if no t
With that translation (x,1/2,1/2) lots of your reflections with k and l
Odd will be relatively weak and those zones usually have higher r factors.
Look at the plots of reflection zones in hklview and you will probably
notice weak and strong zones.
The translation will make selecting the soacegrou
Hi Cat,
If your pseudo-centering actually leads to significant change in your
cumulative intensity distribution, you might have a convincing explanation as
to why the R-values would remain on the high side. I had a pseudo-body centered
case a long time ago (https://doi.org/10.1107/s09074449
Hi,
I have collected data for a protein which solved easily to 1.6 Å using MR.
However, during data analysis and refinement it clearly has translational NCS.
According to Xtriage on Phenix:
Frac. coord.: 0.072, -0.498, 0.5
Distance to origin: 68.798
Height relative to origin: 51.479%
p-value (he
Join us for this International Summer school as we explore exciting
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Crystallography Synergy workshop”
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the strengths of both techniques: Cry
