Sorry did not mean to go off topic. Original question was requests for
suggestions of cleavable c-term tag vectors. I only meant to provide info on
one type recommended by another. Others may have further suggestions for Flavio.
From: CCP4 bulletin board on behalf of Karla J. F.
Satchell
Date
Hi Gerard,
indeed, thank you for your clarification, I'm rusty since I'm on
something else now.
Anisotropy might not be the right word, as it does mean the opposite of
isotropy.
I meant 2 phenomenon at play: lack of completeness in the high
resolution shells, and different intensity falloff
Hi everyone—
Thought I might chime in.
This technology was developed in my lab based on our studies of toxins and we
have methods papers and two patent.
The CPD-tag works great for production of protein with minimal residual
residues on the protein. Our studies indicate that it can help solubil
Hi Flavio
While i am not aware of any commercial vector with cleavable c
terminal tag, you can easily make your own by introducing protease cleavage
site of your choice. However this strategy will results in quite a few extra
residues from protease site.
An alternative, which is
Dear Vincent,
Thank you for chipping in as you did, with so much useful feedback
about the difficulty of re-using PDB depositions containing anisotropic
data. It is a very useful picture of what is definitely a "bleeding edge" on
the "R" side of the wwPDB's "FAIR" ideal.
It would be mos
Isnt this what you want? output of CCP4I2 fitting buccaneer to the other..
On Fri, 16 Feb 2024 at 09:46, zx2...@connect.hku.hk
wrote:
> Dear all,
>
> Following the advice provided by Paul and Yi Min, the "*Symm Shift
> Reference Chain Here*" function has proven successful!
>
> I am deeply grate
Dear all,
Following the advice provided by Paul and Yi Min, the "Symm Shift Reference
Chain Here" function has proven successful!
I am deeply grateful for your guidance.
Best regards,
Xin
From: zx2...@connect.hku.hk
Sent: 16 February 2024 17:26
To: Paul Emsley
Dear community,
I'm looking for a commercial expression vector for E.Coli with a
cleavable his-tag at the c-ter. Anyone can help me?
Thank you in advance,
Flavio.
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Hi Clemens and all,
I've been following with a lot of interest of course, anisotropy has
taken a lot of space in my membrane-protein crystallography life.
I remember the many exchanges we've had with you and Global Phasing, as
well as many other software developpers over the years.
I would li
