Have you tried sodium?
From: CCP4 bulletin board On Behalf Of Arpita Goswami
Sent: Sunday, December 17, 2023 11:47 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Query on density fitting to phosphate
Dear All,
Hope you all are doing well.
The density in the image (in link below) is fitted wit
Dear Arpita,
The hydrogens on phosphate, just like sodium and potassium, will come off the
oxygens in water.
To be more explicit, you don't have mono- or di-hydrogen phosphate in water
(except transiently), you just have phosphate, depending somewhat on the pH of
course. At 2.5 Angstrom resolut
Hello, it doesn't look like a phosphate very much, to be honest, and being so
close to the carboxylate suggests it isn't. Is there any chance that it is an
alternative conformation of the Arg side chain? If you model it as water, is
there residual positive difference density?
Best wishes, Jon C
Dear All,
Hope you all are doing well.
The density in the image (in link below) is fitted with PO4 ion, although
the crystallization condition has both mono and dihydrogen phosphate which
is not fitting without hydrogen. But the resolution is 2.5 A, so hydrogen
may not be put in, or is there any
