Biochemist Position at Oak Ridge National Laboratory
We are seeking a Postdoctoral Research Associate with expertise in biochemistry
and molecular biology to join an interdisciplinary team in the Neutron
Scattering Division (NSD) at Oak Ridge National Laboratory. You will contribute
to a proje
Debipreeta
There is a fix with instructions on the download page and it is also here
on the Phenix bulletin board (
https://phenix-online.org/pipermail/phenixbb/2023-October/025579.html).
Cheers
Nigel
---
Nigel W. Moriarty
Building 33R0349, Molecular Biophysics and Integrated Bioimaging
Lawrenc
You may as well have fun by manually building your molecule B into 1.9A ed map
when R-factors are already in mid 30s.
Vaheh
From: CCP4 bulletin board On Behalf Of Sam Tang
Sent: Monday, November 6, 2023 2:02 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] RES: [ccp4bb] About model building
Dear all
Thanks again for more input to the question. And credits to Eleanor and Kay
for pointing out the high R-factor and possible issue with the space group.
Their advice prompted me to revisit the MR solution and it happens that
another solution, in P3121, gave a better map with R-factor after
Hi Sam.
If you still have any of your crystals or any protein solution left in the well
you harvested your crystals, I would run a MS/MS with them. Next step would be
to run AF with your known chain A and your best Mass Spec hit (s), and use the
resulting model for MR.
Good luck
Rafael Marqu
It is with much sadness that I write to inform the community that Robert (Bob)
Blessing passed away on October 30th.
Blessing graduated from King’s College in 1962. He received a Ph.D. in
Chemistry from Ohio University, and in the following four years, he carried out
post-doctoral studies at th
Dear All,
We have a vacancy for a Structural Biology Facility Manager at the John Innes
Centre.
The John Innes Centre is an independent, international centre of excellence in
plant and microbial sciences. We nurture a creative, curiosity-led approach to
answering important questions in bioscie
Hi,
If you still have crystals left, you could soak crystals with KI3 and collect
data at Cu wavelength for SAD phasing, which could help you to resolve the
missing piece. Maybe.
Cheers,
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben Gurion University
Beer Sheva, Israel
On Nov 4, 2023 10:0
Yes. I tried to weight those B values by occupancies, but the result is
still not the same as the value reported by refmac5.
Qixu Cai
Email: caiq...@gmail.com
Eleanor Dodson <176a9d5ebad7-dmarc-requ...@jiscmail.ac.uk>
于2023年11月6日周一 14:04写道：
> Are there some atoms with occupancies < 1.0 ?
>
I tried both and none of them is the same as reported by refmac5.
Qixu Cai
Email: caiq...@gmail.com
James Holton 于2023年11月6日周一 01:11写道：
> Are you averaging over all ATOM records? Or are you including HETATM as
> well?
>
> On 11/5/2023 5:45 AM, Qixu Cai wrote:
>
> Dear all,
>
> I found that t
Thanks for your reply. Does the refmac5 program weight the mean B value by
the resolution?
Qixu Cai
Email: caiq...@gmail.com
Ian Tickle 于2023年11月5日周日 19:34写道：
> The arithmetic mean B value from the structure as quoted everywhere is
> pretty meaningless anyway and 10 Ang.^2 either way is proba
Hi Sam,
What you write does not sound good to me.
Did you have Phaser check "all possible [spacegroups] in same pointgroup" (this
is how it is called in ccp4i)? P321 is quite rare, compared to P3121 and P3221.
If you miss a screw axis, you will not find a solution.
An R-factor of 50% does not at
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