Hi, If you still have crystals left, you could soak crystals with KI3 and collect data at Cu wavelength for SAD phasing, which could help you to resolve the missing piece. Maybe. Cheers, Boaz
Boaz Shaanan, Ph.D. Dept. of Life Sciences Ben Gurion University Beer Sheva, Israel On Nov 4, 2023 10:04, Sam Tang <samtys0...@gmail.com> wrote: Dear community, I am solving the structure of a complex between proteins A and B, where A is a protein with known homologs and B is a novel protein isolated from plant. The diffraction data was at 1.9 Ang collected in-house, indexed to P321. Using A as the search model, we have got a reasonable solution where, after one round of refinement, the A chain fits the map pretty well. What's left was to extend the termini and fit a few rotamers. For protein B (B chain) I have tried the web version of ARP/wARP but the outcome was not really good. The model was not successfully built as indicated by low model completeness and score. The tricky thing may be that we do not have the complete sequence information of this protein B in-hand. (The other way round, we more or less wish to rely on the high resolution data to confirm its sequence.) What approach would you then recommend to build the B chain in this scenario? Thanks in advance and best regards, Sam ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
