Dear Colleagues,
We are glad to announce the EMBL Course:
Solution scattering from biological macromolecules
6 – 10 November 2023, Virtual
27 November – 1 December 2023, EMBL Hamburg
The online lectures will take place from 6 – 10 November 2023. In addition, a
small number of selected participa
a possibility, if you must try, would be to look at intein-based protein
ligation. Hideo Iwai has a series of papers on this over the last 15+ years.
Adrian
On 26 Jul 2023, at 11:14, Armando Albert
mailto:xalb...@iqfr.csic.es>> wrote:
Dear all,
We are trying to characterize a protein consistin
Hi Armando,
Have you looked at predicted models e.g. Alphafold for the full-length protein?
+ Are there experimentally determined structures for full length structural
homologues or orthologues from other species to validate computational models?
You may be able to align your domains against th
Hi Armando
At the risk of appearing flippant, why not submit the sequence of the
full-length protein to something like Alphafold (readily available to all via
ColabFold (see
https://colab.research.google.com/github/sokrypton/ColabFold/blob/main/AlphaFold2.ipynb)?
I suspect that each of the t
Dear all,
We are trying to characterize a protein consisting of two domains. We have
successfully produced, purified and crystallized both domains independently.
However, we are unable to overexposes a soluble form of the full-length
protein. Can anyone provide a strategy to merge back the ind
