[ccp4bb] AW: [ccp4bb] dimeric tag to induce the homodimerization of protein

Hi Dhiraj, you could also consider making a fusion protein of your protein with itself, with a suitable long linker (gly-ser-gly-ser etc.?) in between. At least that dimer won't dissociate. Best, Herman Von: CCP4 bulletin board Im Auftrag von Srivastava, Dhiraj Gesendet: Mittwoch, 23. Septembe

[ccp4bb] EVENT DATE CORRECTION: Stanford-SLAC CryoEM Center (S2C2) Workshop on CryoEM of COVID-related Macromolecules, September 28, 2020

S2C2 Workshop - CryoEM of COVID-related Macromolecules, September 28, 2020 8:00 AM- 1:00 PM (PDT) Held as part of a suite of workshops during the SSRL/LCLS Annual Users' Meeting, this session highlights the recent use of cryoEM structure determination of SARS-COVID-2 related macromolecules. S

[ccp4bb] Stanford-SLAC CryoEM Center (S2C2) Workshop on CryoEM of COVID-related Macromolecules, October 28, 2020

S2C2 Workshop - CryoEM of COVID-related Macromolecules, October 28, 2020 8:00 AM- 1:00 PM (PDT) Held as part of a suite of workshops during the SSRL/LCLS Annual Users' Meeting, this session highlights the recent use of cryoEM structure determination of SARS-COVID-2 related macromolecules. Spe

[ccp4bb] Information, Resolution, FSC, ... All you ever wanted to know!

Dear All, We have just posted an important paper on Information Theory in the context of Harvesting new Information in the imaging sciences, in (arXiv)! Any image one collects in a microscope, or 3D image in X-ray tomography, X-ray crystallography, for example, is covered by this comprehensive

Re: [ccp4bb] dimeric tag to induce the homodimerization of protein

Thanks everyone for all the nice suggestions. regarding Matthew's question, Yes, There is a possibility of polydispersity due to the proteins making chain/aggregate, however the affinity between the dimeric protein A and monomeric protein B is poor and kinetics of dissociation is very fast thus

[ccp4bb] Postdoctoral position at CBI / IGBMC, Strasbourg

Dear all, we have an open position for a post-doctoral researcher to join the Klaholz group at the Centre for Integrative Biology, IGBMC, Illkirch, France http://igbmc.fr/Klaholz We are studying the structure and function of large nucleoprotein complexes (ribosome complexes, chromatin co

[ccp4bb] Updated Validation Reports for Released PDB Structures

Dear CCP4BB All validation reports for released PDB entries have been updated and are now available with recalculated percentiles. In addition, the validation reports now include 2D diagrams highlighting ligand quality, map and model fit for EM entries, and calculated map coefficients used for

[ccp4bb] open Post-Doc position in Amsterdam

Hi everyone, we have an *open Post-Doc position* (1+2 years) together with our collaboration partner at the University of Amsterdam (UvA). The project will run at the UvA, the structural work will be done here in our facilities at the Vrije Universiteit Amsterdam (VU). *Application deadline i