Dear Venkata,
Check with Birkbeck online crystallography certificate course here:
http://www.bbk.ac.uk/study/2020/postgraduate/programmes/TPCCRPRO_C/.
Best wishes,
Natesh
On Wed, 22 Jul 2020 at 09:22, venkata narayana Are <
venkat.sai.biochemis...@gmail.com> wrote:
> Dear Sir,
>
> My self Venka
Dear Sir,
My self Venkat completed Ph.D. in Biochemistry (structural biology) later
on joined in a short project where I learned cell biology and virology. I
have solved 15 structures during my Ph.D., further I wanting to learn
membrane protein receptors structural biology and other complex protei
Hi REFMACers:
To get the citation information of REFMAC, I made a test run, which gave me:
==CCP4 7.0.076: Refmac version 5.8.0253 : 06/20/19
...
"Overview of refinement procedures within REFMAC5: utilizing data
from different sources"
O.Kovalevskiy, R.A.Nicholls,
Dear Bob,
Many thanks for posting this memory of Ward. It was such a sad shock to learn
of his passing. Like many of the newsgroup readers I remember Ward as an
outstanding colleague and program manager. It was my pleasure to talk with him
regularly as my point of contact for Phenix and ALS-E
Hello Juan
First, there's a phenix.refine bulletin board, on which you might
attract the attention of the developers, which might help.
http://www.phenix-online.org/mailman/listinfo/phenixbb
I've been using 1.17-3644 without issues after transitioning from
something older. Consider downgradi
Dear all,
I have been working on the refinement of a crystal structure using
phenix.refine from the 1.12-2828-Intel-Linux-2.6 version of Phenix. I
have recently replaced my computer by a MacBook and I have upgraded
Phenix to the 1.18.2-3874-MacOs version. However, I found that the
refineme
Hi All,
We are hiring! We are seeking a highly motivated and skilled (Senior) Scientist
with experience in programming pipelines, biostatistical analysis and data
modelling to join Lonza's Applied Protein Services (APS) Bioinformatics team
based in Cambridge, UK.
To apply:
https://pharma.lonza
Hi Samer,
(1) Given that you say there is a relatively high concentration of Zn2+ ions in
the crystallisation buffer, I suspect this might be your candidate.
(2) If the Zn2+ ion is correctly represented in the pdb file, your refinement
program of choice will know what the target co-ordination d
Hello,Thank you for your kind reply.In this case that could be either Zinc or
Nickel. Sorry, I should've mentioned how I purified the protein complex.
The protein is secreted in HighFive cells, to purify it by its 6xHis tag, I
used Ni sepharose excel beads, eluted with Imidazole and then furthe
Hello,Thank you for your kind reply.If the distances are still less than 2.2Å,
would coot and Refmac still consider that as a clash. When I try to fit in
Imidazole, even if the distance is more than 2.8Å, it is still considering it a
clash.Sorry, I should've mentioned how I purified the protein
Hello,Thank you for your kind reply.We used glycerol to fish out the
crystals.Sorry for not including more info before, I thinkI should have
mentioned how I purified the protein complex.
The protein is secreted in HighFive cells, to purify it by its 6xHis tag, I
used Ni sepharose excel beads,
Hello,Thank you for your kind reply.Do I need to link them together in the
structure? Is there a method for that?
>From the other replies I thought I should mention how I purified the protein
>complex.The protein is secreted in HighFive cells, to purify it by its 6xHis
>tag, I used Ni sepharos
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