Re: [ccp4bb] Figure of merit in refinement

2019-10-02 Thread Andre LB Ambrosio
Dear Jonathan, many thanks for this. I will have a look at it right away. With best wishes, Andre. On Wed, Oct 2, 2019, 7:51 PM Jonathan Cooper wrote: > This is a very good place to start: > > https://www-structmed.cimr.cam.ac.uk/Course/Statistics/statistics.html > > Also recommend this one: > >

Re: [ccp4bb] Figure of merit in refinement

2019-10-02 Thread Jonathan Cooper
This is a very good place to start: https://www-structmed.cimr.cam.ac.uk/Course/Statistics/statistics.html Also recommend this one: https://doi.org/10.1107/S0108767386099622 and Main, P. (1979) Acta Cryst. A35, 779-85 - the maths in this one are a bit easier! On Wednesday, 2 October 2019,

[ccp4bb] Figure of merit in refinement

2019-10-02 Thread Andre LB Ambrosio
Dear all, How is the phase error estimated for any given reflection, specifically in the context of model refinement? In terms of math I mean. How useful is FOM in assessing the phase quality, when not for initial experimental phases? Many thank in advance, Andre. #

[ccp4bb] PhD position in the Chakrabarti group at Freie Universität Berlin, Germany

2019-10-02 Thread Sutapa Chakrabarti
3-year PhD position at the Freie Universität Berlin Dear All, A funded PhD position is available in the lab of Sutapa Chakrabarti (at the Institute of Chemistry and Biochemistry, Freie Universität Berlin, Germany) to work on the coupling of mRNA decay and translational repression in eukaryotes

[ccp4bb] Assistant Professor Position at UC Davis

2019-10-02 Thread James Anthony Letts
Dear Colleagues, I wanted to bring this great opportunity to join us at UC Davis as an Assistant Professor to the communities attention (https://recruit.ucdavis.edu/JPF03095): The Department of Molecular and Cellular Biology, in the College of Biological Sciences, University of California, Davi

Re: [ccp4bb] Propeptide cleavage for complex assembly

2019-10-02 Thread Darren Hart
Influenza polymerase heterotrimer expressed in insect cells (MultiBac technology) as a fully synthetic polyprotein with subunits separated by TEV sites TevProtease-Tev-PA-Tev-PB1-Tev-PB2-Tev-CFP Reich et al. Nature 2014; doi:10.1038/nature14009 /Extended Data Figure 1 | Production and char

[ccp4bb] Propeptide cleavage for complex assembly

2019-10-02 Thread Paula Salgado
Dear colleagues We have been working on a protein that is produced as a pro-peptide, cleaved internally and reassembled into a complex. The interacting regions are the new C and N-termini at the cleavage site, so no rearrangement or loss of part of the protein is involved. The resulting interac

[ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] no tNCS and Phaser manuals

2019-10-02 Thread Herman . Schreuder
Dear Iracema, Thank you very much for your hint of using CCP4i, I never use it but this time I should have used it to find out how to switch off the tNCS. Unfortunately, I cannot reach any of the phaser.cimr sites. I get the error message that our proxy server does not react. I can reach the ph