Re: [ccp4bb] suggestions for cryoprotectant

2018-10-20 Thread Joseph Brock
Dear Friduous, This is also a good paper to look at once you know your crystals diffract at room temperature: https://www.ncbi.nlm.nih.gov/pubmed/10944336 Many of the salts in your condition will be cryo-protective if you increase their concentration to saturated levels. Cheers, Joseph.

[ccp4bb] Fw: Postdoctoral Fellow Position in Structural Biology

2018-10-20 Thread Vijay Parashar
Postdoctoral Fellow Position Parashar laboratory at University of Delaware College of Health Sciences, is currently seeking a Postdoctoral Fellow to perform structure-function studies of proteins regulating bacterial signaling.  Qualifications:·  Ph.D. in Biochemistry, Molecular Biology, Stru

Re: [ccp4bb] suggestions for cryoprotectant

2018-10-20 Thread Thomas, Leonard M.
This is the first thing you need to do before going down the rabbit hole looking for a cryo protectant. Mitegen make it much easier then in the 'old days' of capillaries. Good luck, Len lmtho...@ou.edu On Oct 20, 2018 2:02 AM, Andreas Förster wrote: Dear Firdous, get some Mitegen sleeves an

Re: [ccp4bb] suggestions for cryoprotectant

2018-10-20 Thread mesters
Hi, have a look at the following interesting papers https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466044/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852300/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252874/ Good luck J. Am 19.10.18 um 23:57 schrieb Firdous Tarique: Dear members I hav

Re: [ccp4bb] suggestions for cryoprotectant

2018-10-20 Thread Andreas Förster
Dear Firdous, get some Mitegen sleeves and test your crystals at RT. Before you establish diffraction, there’s no need to agonize about cryo-conditions. Good luck! Andreas On Fri, 19 Oct 2018 at 23:57, Firdous Tarique wrote: > Dear members > > I have got beautiful crystal hits in SaltRx scr