Not being in a model does not show that it's really not there.
This is especially true when considering crystals that diffract to rather low
resolution (~2.8 - 3.5+ Å).
You shouldn’t expect in that case to uniquely identify very many buffer
components.
Diana
**
Hi Tobias,
I don't know of any specific claims made about this but you'd better check the
data yourself if you find an example. A nice paper by Bill Hunter and his team
(http://scripts.iucr.org/cgi-bin/paper?S1744309111005835) showed a case where
Co2+ was an essential ion for crystallisation ,
Not quite what you were looking for, but there was just that paper from
Alexander McPherson abut dye binding. They show that some of the dyes seem
to bind really tight to the protein, but are invisible in the structure.
Maybe there are also some interesting references in there. Sorry haven't
got ar
Thanks for the replies I have received so far (on and off list).
I would like to emphasize an important aspect: What about crystallization
trials where the ingredients do not show up, but cannot be omitted for the
crystallization, e.g. buffers to control pH, as mentioned in one response -
I am als
Dear all,
I would like to bring to your attention the following opening.
Sincerely,
Yves Muller
Osnabrück University's School of Biology/Chemistry invites applications
for the following professorship to start as soon as possible:
Structural Biology (Bes.Gr. W3)
We are looking
Dear all,
I am looking for some general references regarding the fact that for a
crystallization condition not all ingredients of the crystallization
cocktail will show up in the crystal structure. Ions could be disordered,
buffer components located in the solvent regions, etc.
I think this is ra
