Re: [ccp4bb] precision protease plasmid

2018-06-20 Thread Neeraj Kr. Mishra
Hello BBians, sorry for the off-topic question. I have plasmid which possesses the precision protease site to get rid of his-tag. Does anybody have precision protease plasmid? If someone has please share with me. I will be very thankful to you. On Wed, 20 Jun 2018 at 10:25, Neeraj Kr. Mishra wro

[ccp4bb] Postdoctoral position in epigenetics at UCSF (San Francisco, USA)

2018-06-20 Thread Danica Fujimori
Position description: The research group of Dr. Danica Fujimori at University of California San Francisco is seeking highly motivated postdoctoral candidates with a background in biochemistry, structural biology or chemical biology to join our research group. The broad interests of the lab are i

[ccp4bb] Regarding symmetry notion in coot

2018-06-20 Thread Anurag Misra
Dear all, What is the meaning of the last field — the one in curly brackets -- that describes the symmetry transformation of a given molecule in coot? For example, for a given X Y Z, coot displays the symmetry transformation of an equivalent position as " -X, Y+1/2, Z + (0 -1 1) & { 1 0 1 }”. Can

[ccp4bb] Fwd: Senior Laboratory Technician - Protein Purification - in the Medicines Discovery Institute at Cardiff University.

2018-06-20 Thread benjamin bax
We are advertising for a Senior Laboratory Technician Protein Purification in the Medicines Discovery Institute at Cardiff University, UK. The goal of the newly-established Medicines Discovery Institute in Cardiff is to translate world-leading research on scientific understanding of disease

[ccp4bb] Postdoctoral position in membrane protein structure and function at the NIH

2018-06-20 Thread Anirban Banerjee
There is an open postdoctoral position for a membrane protein structure-function project in my lab at the NIH. We are currently focusing on integral membrane enzymes (Science. 2018, doi: 10.1126/science.aao6326) and transporters of transition metal ions (J Biol Chem. 2018, doi: 10.1074/jbc.M117.81

[ccp4bb] Postdoc Position in Structural Biology in Berlin

2018-06-20 Thread Ursula Neu
The “Structural Glycovirology” group at Freie Universität Berlin in Berlin-Dahlem is looking for an enthusiatic postdoctoral scientist with a strong background in structural biology, holding a PhD in Biochemistry, Biology or related subjects. Carbohydrates densely cover every living cell and thus

Re: [ccp4bb] PanDDA error revisiting old run

2018-06-20 Thread Frank von Delft
Hi Chris - that said, it is probably a better idea to repeat the old analyses with the new version, even if that costs more time. Frank On 20/06/2018 11:42, Pearce, N.M. (Nick) wrote: Hi, Yes, it is the case that newer pandda version may not be compatible with old pandda analyses. You’ll ha

Re: [ccp4bb] PanDDA error revisiting old run

2018-06-20 Thread Pearce, N.M. (Nick)
Hi, Yes, it is the case that newer pandda version may not be compatible with old pandda analyses. You’ll have to downgrade to the version you used originally. Thanks, Nick > On 20 Jun 2018, at 12:17, Chris Richardson wrote: > > One of our group has been using the newly updated PanDDA (0.2.12

[ccp4bb] PanDDA error revisiting old run

2018-06-20 Thread Chris Richardson
One of our group has been using the newly updated PanDDA (0.2.12 in ccp4 7.0.058) to look at some data previously processed with PanDDA. It dies with an error on unpickling a pickle file (see errors below). My guess is that changes have left it unable to read some pickle files from previous ve