-- Forwarded message --
From: Daniel M. Himmel, Ph. D.
Date: Wed, Mar 7, 2018 at 12:23 AM
Subject: Re: [ccp4bb] suggestions on a microscope for Crystallography
To: Chandramohan Kattamuri
My best experience was with Leica microscopes, such as the S6E.
Leica microscopes have optic
Hi
I'm looking for suggestions on a good microscope for looking at crystals, which
includes polarization, light source (fiber optics), crosshairs and camera
mount. What Models and make?
Thanks in advance
Chandra
Hi Rajesh,
It might be water molecules present at the symmetry axis, one more water
molecule at the centre (exactly at the axis, with occupancy of 0.5). If you
put three water molecule you probably may find the other three
symmetry-mates water molecules in opposite sides. OR One water molecule at
Hi Rajesh,
You may find this database is also useful while refining your model
http://mespeus.bch.ed.ac.uk/MESPEUS_10/
and for calcium
http://mespeus.bch.ed.ac.uk/MESPEUS_10/_5.jsp
Abhik
On Tue, Mar 6, 2018 at 10:59 PM, Patrick Loll wrote:
> Calcium likes to form octahedral complexes with w
Calcium likes to form octahedral complexes with water (or other
oxygen-containing) ligands. This looks like a classic example.
After you model and refine this, you’ll want to check water-metal distances, to
make sure they are appropriate for calcium. There is a nice literature on such
things,
You could try to put CA (as you have CA in your crystallisation condition) and
waters for coordinating atoms. If after refinement distances are around 2.3-2.4
(if I remember correctly) and B values of atoms (CA and waters) are consistent,
i.e. similar to each other then CA is good interpretation
Hi Rajesh,
Can you please let everyone know the buffer/reagents involved throughout the
process.
Also, how strong is the density? In other words, until what contour level you
see it in the Fo-Fc map?
Anyways, looking at the shape, it looks well coordinated - could be cacodylate?
Or water, am
Hi Hussain,
Crystallization condition is 200 mM CaCl2 and 20% PEG 3350.
I some what realized that it could be Ca++ with coordinated waters. But I
really wanted to have
second opinion from our group.
Thank you every one.
-Rajesh
On Tue, Mar 6, 2018 at 5:37 PM, Hussain Gps wrote:
> Hi Raje
Mg2+(H20)6 ?
> On 6 Mar 2018, at 22:19, Rajesh Kumar wrote:
>
> Dear All,
>
> Have you had experience with this kind of density? I am wandering what this
> could be?
>
> Thank you very much for the help.
>
> -Rajesh
>
>
>
>
It looks like 6 coordinated metal atom with coordinated (possibly) with waters.
The simplest case could be NA. You could use anomalous difference map to see if
there is any anomalous signal there.
Regards
Garib
On 6 Mar 2018, at 22:19, Rajesh Kumar wrote:
> Dear All,
>
> Have you had experi
Last update...
Everything works well with the same files and the same Coot version on an
other computer. The problem may come from the os of my computer which is
too old and has to be upgraded.
Thank you for your help.
2018-03-06 9:20 GMT+01:00 M T :
> Dear all,
>
> I did something very simple.
Dear all,
This email is sent on behalf of Professor Dale Wigley, Imperial College London,
who is looking to recruit two Research Associates. The full details are
attached, see the following for brief info.
The successful candidates will join a multi-disciplinary team of international
researche
Dear all,
A postdoctoral position is available in my lab in the Department of
Neuroscience, Physiology & Pharmacology at University College London.
The post is to investigate how calmodulin-dependent protein kinase II (CaMKII)
is targeted in neurons, which underlies its role in strengthening s
Dear all,
I did something very simple...
Starting from the pdb I obtained from PRODRG server (manual drawing, then
transfer to PRODRG and download of the pdb from server), in CCP4 I used
ProDrg to generate a new .cif and a new .pdb.
After I used "View Job Results (ew style)" to open my new pdb in
14 matches
Mail list logo
