[ccp4bb] How to define the DNA bond between P and O3’ from two different symmetric units?

Hello, everyone， Recently, we solve a protein-DNA complex. 20bp-DNA was used for crystallization, but only 6bp was found in one symmetric unit. My question is： How to define the DNA bond between P and O3’ from two different symmetric units during my refinement? Peng

Re: [ccp4bb] Automatic restraints in Refmac too loose?

Hi Maria, What is the refmac version? It should be on the top of the log file. Regards Garib On 29 Jan 2018, at 17:03, Maria Håkansson wrote: > Hi, > > I have a question regarding the automatic restraints in Refmac5 and > CCP4Interface 7.0.050. > > For a few years I have relied on automati

[ccp4bb] Automatic restraints in Refmac too loose?

Hi, I have a question regarding the automatic restraints in Refmac5 and CCP4Interface 7.0.050. For a few years I have relied on automatic restraints settings in the CCP4I interface which usually resulted in r.m.s. bond deviations of about 0.02 Å and good convergence of refinement (lower R-fact

[ccp4bb] AW: [ccp4bb] Publishing structure of a protein

Dear Raj, The pdb will accept any crystal structure submitted to them. They do not have editors and referees that refuse to accept crystal structures. In the worst case, your structure may have validation problems and you will get questions about it. But this will happen with published crystal

[ccp4bb] XChem user progamme: recruiting staff

Dear MXers We are recruiting two scientists to Diamond's XChem facility to expand its user programme for crystallographic fragment screening. The facility, set up at beamline I04-1 in partnership with the SGC-Oxford, is now rout