[ccp4bb] research tech position, Brown University

2017-08-07 Thread Deaconescu, Alexandra
Dear colleagues, I have one research assistant position available in my lab at Brown University in Providence. Rhode Island. Please apply using this link , but feel free to email me

Re: [ccp4bb] refmac output

2017-08-07 Thread Eleanor Dodson
the CCP4 gui does just that - provides map coeffs by default.. You can seek out the full REFMAC output if needed but it is not there by default. Eleanor On 3 August 2017 at 02:04, Pavel Afonine wrote: > Hi Ed, > > your suggestion makes perfect sense to me, and it's trivial to add an > option to

Re: [ccp4bb] doubt regarding setting resolution cutoff

2017-08-07 Thread Eleanor Dodson
First comment - your data looks excelent - even to 1.8A. Second - look also at the wilson plot and second moment plots - these should not be jerky - wilson plot falling off smoothly, 2nd moment pretty constant to the good resolution limit.. Thirdly - just use all your data and check the refinemen

Re: [ccp4bb] Some TLS issues in REFMAC5

2017-08-07 Thread Eleanor Dodson
Oh dear - TLS transformations are a bag of worms! I usually start all over again if I want TLS .. SET all Bs to B_wilson Run 10 cycles of TLS with the groups listed in the PDB header. That is boring but comparatively safe! Eleanor On 6 August 2017 at 15:36, Stefano Trapani wrote: > Dear a

[ccp4bb] Chromatography System for protein purification

2017-08-07 Thread Muhammad Imran
Hi everyone, I will be grateful to all of you if you can help us in choosing chromatography system. Since new companies (Bio-Rad and Knauer) are also offering systems which earlier were (Amersham/GE-AKTA) specialty. However we do not have any experience of these system except Akta. If any one has