Dear Abishek,
shadows should be excluded from processing since the outlier rejection
algorithms cannot distinguish between weak and shadowed reflections!
In XDS, you could use a generous static mask (UNTRUSTED_*), or a dynamic mask
as specified by FILTER.HKL.
HTH,
Kay
On Thu, 27 Apr 2017 2
Dear Abhishek,
This looks very like the goniometer shadowing we have seen from the mini Kappa
goniometer on I04 at DLS. DIALS (and xia2) now support dynamic shadow masking,
although a model for the goniometer shadowing needs to be coded in the detector
"format class" in order for it to work. We
Depends on the geometry of the goniometer/pin relative to the X-ray beam.
If the pin is too close, it will possibly cast a shadow. If the pin is
aligned at a shallow angle relative to the X-ray source beam, the pin or
the goniometer may get in the way regardless of how far away the crystal is
from
If it moves it’s the shadow of the Kappa head.
Edward Snell Ph.D.
President and CEO Hauptman-Woodward Medical Research Institute
Assistant Prof. Department of Structural Biology, University at Buffalo
700 Ellicott Street, Buffalo, NY 14203-1102
hwi.buffalo.edu
Phone: (716) 898 8631 Fax
This looks like the cryo-stream is too close to your crystal. I have seen this
before on our home source.
Dr. Stehen Keable
Montana State University
> On Apr 27, 2017, at 2:49 PM, Dr A.A. Jalan wrote:
>
> Dear all,
>
> The attached diffraction image has a shadow at the right edge. It moves
The Iverson laboratory at Vanderbilt University has several immediate openings
for qualified postdoctoral associates interested in investigating broad themes
underlying how proteins encode information. Positions could be associated with
one of three possible projects:
1. Characterization of hos
Hello Everyone,
We included 10 mM EDTA in our Ni-NTA and gel filtration buffers to prevent
aggregation of our protein, as suggested by the literature (indeed,
excluding EDTA led to precipitation during the concentration steps). In the
presence of 10 mM EDTA, we were able to concentrate the protein
Dear all,
Details of the 25th Protein Structure Determination in Industry Meeting, to be
held at Cambridge UK in November this year, can be found below
http://www.psdi2017.org/home
Best wishes
Pamela
-
Pamela Williams, DPhil
Director, Molecular Sciences
Astex Pharmaceuticals Ltd.
4
Dear colleagues-
I would like to bring to your attention an opening for a Scientific Computing
Specialist at the Rockefeller University. This is a full-time staff position
focused on providing computational support for cryo-EM efforts. Please feel
free to direct any informal inquiries to me at
Have you looked at a self rotation function? Is there a clear 3-fold axis
perhaps?
Eleanor
On 27 April 2017 at 10:45, Mark J van Raaij wrote:
> Does the self-rotation function suggest presence of NCS axes? If so, this
> may help you figure out the symmetry inside the a.u..
> If you haven't done
Dear All,
Apologies that this is not directly CCP4 related, but over the years it has
been an excellent medium for contacting high-performance compute specialists...
I'd like to advertise the availability two exciting and challenging new
opportunities to work in research computing/high-perform
Does the self-rotation function suggest presence of NCS axes? If so, this may
help you figure out the symmetry inside the a.u..
If you haven't done so already, try diffracting a crystal at room temperature,
to make sure cryo-protection and freezing did not affect the diffraction.
In any case, at
Dear all,
sorry for off topic question.
May i know if anybody uses homemade silinization of coverslips for protein
crystallization purposes?
I have purchased Sigmacote SL2-100 ml for silanizing coverslips for hanging
drop protein crystallization setup.
Please share your methods to siliconize co
Dear Jademilson,
At a CCP4 APS workshop a few years ago, one of the
students solved a molecular replacement problem where I think there were close
to 40 copies in the asu. I have to say that many people were surprised by this,
but I think the protein was smaller and
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