Dear all,
this all depends on the protein: some 7TM proteins are nicely resolved
as 1 band of ~the expected size upon 5 min sonication - 5 min 95 °c - 5
min sonication in sample buffers containing 2 M urea
Others still gave mono- & dimers
The treatment above also wored for a pentameric 4TM pr
Hi Alun and Kay,
PDB_REDO does some more extended twinning testing with among others Phaser, so
it would be worthwhile to have a look at that. On the server it refuses to do
twinned refinement if there is no clear indication of twinning even if the user
assumed twinning before.
The fact the
Hi Alun,
1. What does phenix.xtriage think of the dataset?
2. What kind of redundancy do you have in the dataset?
3. Sometimes with a large crystal and a small beam the twin fraction varies as
you rotate the crystal and illuminate different parts of the crystal (which can
have different twin fra
Dear Bert
You made the comment a few weeks ago not to boil helical membrane proteins
for SDS-PAGE. Could i please ask, does this also apply to type I membrane
proteins that only have a single a-helix, or is it just membrane proteins
that are predominantly helical?
Thanks
Rich
On 22 February 2017 a
On Mar 1, 2017, at 8:19 PM, Shaun Lott
mailto:s.l...@auckland.ac.nz>> wrote:
They diffracted, but weren't much use to us in the end, as they were hard to
reproduce or optimise. Hopefully you have more luck than we did!
This sometimes happens with extremely soluble proteins. If these are the o
We have seen a similar thing once, where we obtained proteins crystals simply
by dehydration of the drop:
Sharpe, M. L., Baker, E. N., and Lott, J. S. (2005) Crystallization of a
protein using dehydration without a precipitant. Acta Crystallogr Sect F Struct
Biol Cryst Commun 61, 565–568.
They
Dear colleagues,
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Hi Alun,
that's difficult to understand for me. It is my understanding that for an
imperfect model, refmac will indicate a twin fraction >0 even if in reality it
is 0. But alpha=0.372 sounds too high for that.
My questions would be -
a) is it a single dataset, or did you merge ? If the latter, i
Hi Everyone,
I have a (2.5 - 2.3 Angstroms) data set the process as I4 or I222.
Aimless indicates twinning in both space groups and all the lower
symmetry space groups consistent with the reduced cell. Molecular
replacement works in I4 but not I222 etc. I can see new density for a
ligand we a
For historical perspective:
Molecular structure determination by electron microscopy of unstained
crystalline specimens
PNT Unwin, R Henderson - Journal of molecular biology, 1975
http://www.sciencedirect.com/science/article/pii/0022283675902120
From: C
There are now quite a few structures solved by ED of 3D crystals, esp by Tamir
Gonen's group here at Janelia, and a quick count in the pdb yields ~15
3D-crystal ED structures, some of which are not released. They're getting
better at it all the time. I guess the news has not spread as much as I
Dear Jacob, dear TO,
(I have wondered what can be 'micro' about diffraction - would you know?)
it would be very exciting to get the structure by electron diffraction - as
much as I know it would be the first new structure solved from a 3D crystal
with electron radiation in the PDB. You would ha
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Deart Tim and Kamel,
Thank you for your valuable advice. As Tim pointed out, the Shelx2map
worked perfectly fine, and these are indeed very useful tools for anyone
who is moving the map from the Shelx to pymol or CCP4.
Yes, i did try the COOT exported map extension to .ccp4, but it did not
work fo
Hi Appu,
Did you change the file extension of the exported map extension to .ccp4
(instead of .map)? That worked for me.
Cheers
Kamel
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Paul
Emsley
Sent: 28 February 2017 22:57
To: ccp4bb
Subject: Re:
Dear all,
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