Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

2017-02-16 Thread wangsa tirta Ismaya
We used cOmplete Histag column from Roche, 5 ml prepacked. We bought 3-4 prepacked columns and a bottle of 25 ml (cheaper). The nice thing about this Roche pack, if you closed the outlet and let the pump run into the inlet, the cap will slowly detach .. We use this trick to replace the resin in

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

2017-02-16 Thread Thomas Cleveland
I'll second those suggestions and also note that empty XK columns from GE regularly show up on eBay for pennies on the dollar. Roch cOmplete is really great in situations where there are chelators around (especially proteins secreted into eukaryotic tissue culture media). It saved me tremendous am

[ccp4bb] Post-doctoral Research Associate position in Structural Vaccinology

2017-02-16 Thread Thomas Krey
The “Structural Virology” group located at the Institute of Virology, Hannover Medical School, invites applications for a DFG-funded Post-doctoral position (Structural Biology / Vaccinology / Virology) within a joint project involving EPFL Lausanne (Switzerland), Twincore and Hannover Medical S

Re: [ccp4bb] off topic: Alternatives to GE HiTrap columns

2017-02-16 Thread David Blum
We have had good luck with columns from Essential Life Solutions ( http://www.essential-life.net/). They are easy to work with and hold up under pressure well. Best, David -- David L. Blum, Ph.D. Director, Bioexpression and Fermentation Facility Department of Biochemistry and Molecular Biol

[ccp4bb] Post-doctoral Research Associate position

2017-02-16 Thread Faiz Mohammed
Dear All, *Post-doctoral Research Associate position available for crystallographer/protein chemist with molecular biology experience **at the CCMSB, Case Western Reserve University.* We are looking for a candidate with experience in protein crystallography to work on a project involving struct

Re: [ccp4bb] Composit omit map vs. ligand

2017-02-16 Thread Gerard Bricogne
Dear Pavel, Thank you for your reply. I would have thought that many of your users in the drug discovery field would have been aware of it, as many of them use both Phenix and BUSTER. An even more explicit and detailed description of that feature, dated March 2011, may be found at

Re: [ccp4bb] Composit omit map vs. ligand

2017-02-16 Thread Pavel Afonine
Dear Gerard, Thank you so much for letting us know of the reference and details about your long-standing BUSTER method for ligand density maps which we now see is very similar to our recent polder map method. We apologize for not being aware of the early reference and we will be very sure to cite

Re: [ccp4bb] Composit omit map vs. ligand

2017-02-16 Thread Gerard Bricogne
Dear Pavel, As much as I would have wished to, I was unable to contribute to this thread for the past week because of more pressing matters. I hope it hasn't entirely gone cold in the minds of CCP4BB readers. I want to congratulate the Phenix developers for having come up with such a ca

[ccp4bb] Announcement: Cryo-EM School in Italy

2017-02-16 Thread Federico Forneris
Dear Colleagues, we are pleased to announce the 2017 edition of the Italian Association for Crystallography (AIC) International Crystallography School (AICS2017). This year's school title will be "Bridging the gap between cryo-EM and crystallography". The school will be held in Pavia, Italy, f

[ccp4bb] PhD position AND postdoc position available

2017-02-16 Thread Sonja Lorenz
Dear colleagues, We invite applications for a PhD position and a postdoc position in the research group of Dr. Sonja Lorenz at the Rudolf Virchow Center for Experimental Biomedicine, Würzburg, Germany. The Lorenz lab explores the catalytic and regulatory mechanisms of ubiquitin ligases an

Re: [ccp4bb] jhbuild system on Mac

2017-02-16 Thread Tristan Croll
Well, that's nice and straightforward. Thanks! On 2017-02-16 10:15, Stuart McNicholas wrote: I set DYLD_LIBRARY_PATH on the command line to point to location of the svn libraries. On 16 February 2017 at 10:09, Tristan Croll wrote: Hi all, While using the ccp4-jhbuild system to rebuild speci

Re: [ccp4bb] jhbuild system on Mac

2017-02-16 Thread Stuart McNicholas
I set DYLD_LIBRARY_PATH on the command line to point to location of the svn libraries. On 16 February 2017 at 10:09, Tristan Croll wrote: > Hi all, > > While using the ccp4-jhbuild system to rebuild specific components on the > Mac, I'm running into the bug detailed at https://bugs.launchpad.net

[ccp4bb] jhbuild system on Mac

2017-02-16 Thread Tristan Croll
Hi all, While using the ccp4-jhbuild system to rebuild specific components on the Mac, I'm running into the bug detailed at https://bugs.launchpad.net/bzr-mac-installers/+bug/1244668 whenever 'bzr info' is run. The workaround described there no longer appears to to work for OSX Sierra. Before

[ccp4bb] Application Specialist - electron microscopy (TEM) Copenhagen

2017-02-16 Thread Guillermo Montoya
Dear all, Just want to call your attention for the are position open for an Application Specialist in electron microscopy. For those interested , please have a look at the links below. http://employment.ku.dk/staff/?show=889654 http://www.cpr.ku.dk/ http://cfim.ku.dk/ Informal enquiries ab