[ccp4bb] Fwd: CANES MSc/PhD Applications Reminder: Deadline approaching

Applications are now open for the CANES Programme (September 2017 entry) Download our poster here

Re: [ccp4bb] Manual His-Tag Purification

Thanks everyone! Thanks Prof. David Blum. I will do batch load and check. Yours Sincerely, Sundaram.S On Wed, Feb 1, 2017 at 9:06 PM, David Blum wrote: > Hi Sundaram, > > The binding capacity of this column is 40 mg/mL of resin so a 5mL column > will hold a maximum of 200 mg of protein. If y

[ccp4bb] xdsgui errors

Dear all, I'm getting the following errors in terminal while running xdsgui on a mac: Y-COORDINATE (pixel) 2048.0 2048.0 3127.4 3574.5 3127.4 2048.0 968.7 521.5 968.5 NUMBER 2065508618 1163 1065431360718 844 SIGMAB (degree) 0.033 0.034 0.03

[ccp4bb] Postdoc position in cryo-EM - NYC

Dear CCP4ists, My group is seeking highy motiviated applicants to apply for a Post-doctoral Research Associate position. This position, available starting in February 2017, is initially for twelve months with the possibility of further extension. My group studies the structure, function and dyn

Re: [ccp4bb] comparison of different protein states

Hello Guillermo, because you are talking about conformational changes during the catalytic cycle, they should be very conserved along homologous enzymes. Discussing known examples of similar catalytic movements in homologous enzymes would strengthen your argument. During my PhD, I was workin

[ccp4bb] Display issues with CCP4-7 and Coot on Zalman with MacBook Pro

Dear All, Yesterday I started using the new MacBook Pro running on Mac OS 10.12.2, that has only USB-C ports. I also use a Zalman 3D monitor as an additional display with it attached through an adapter. Somehow CCP4 and Coot are not displaying on the Zalman Monitor (the ccp4 and coot windows disa

[ccp4bb] Creating local/specific B-factor restraints in refmac

Hi, As far as I can tell from the refmac documentation, the only way to change B-factor restraints for nearby atoms is by changing the weighting globally, using, e.g., == BFAC 1.0 1.5 2.0 3.0 4.5 == Is there a way to implement a specific local b-

[ccp4bb] Opening for a PhD student at Stockholm University

Posted on behalf of Prof. Martin Högbom Dear colleagues The Högbom lab at Stockholm University has an opening for a graduate student in an ERC-funded project regarding metalloprotein structural biochemistry. Methods include biochemistry, spectroscopy and standard as well as XFEL crystallograp

Re: [ccp4bb] RMSD plot

Dear all, Thanks a lot for all your suggestions. I got it. Thanks again, Madhu. On Wed, Feb 1, 2017 at 11:25 AM, Madhuranayaki Thulasingam < tkmadhu...@gmail.com> wrote: > Dear all, > > I would like to plot RMSD vs amino acids for two superimposed crystal > structures. > > Can anyone suggest m

Re: [ccp4bb] symmetry and pdb remark

It seems the authors have just chosen a different “parent” molecule to submit to the pdb for these two entries, it has nothing to do with the REMARK cards. It would have been “nicer” if they would have submitted the equivalent “parent” molecule, but the unit cells have a relatively big difference