Dear All,
Thank you all for reply.
We have checked the data for twinning.
Our protein is 360 residues around 40 kDa protein.
We have tried TLS refinement.
chain A and B don't superimpose well with chain C and D. (A and B chains
also share slight difference )
Since we don't have proper density for
Hi Tristan,
There are PDB entries that have this, but this makes matters a bit more
complicated in annotation. You have to define LINKs and leaving atoms.
Consistency would be nice here, but that is lacking in these entries.
Cheers,
Robbie
Sent from my Windows 10 phone
Van: Tristan C
The Jackson Lab (http://www.chem.usu.edu/people/faculty/ryan-jackson) at
Utah State University seeks a qualified graduate for a 2017 Presidential
Doctoral Research Fellow position (http://rgs.usu.edu/pdrf/) with a living
stipend of $30,000 to study the structure and function of newly discovered
CRI
I've often wondered about PEG (and, I guess, other synthetic polymers):
wouldn't it just be better to define the monomer, and then model a chain of
however many monomers you need?
T
Tristan Croll
Research Fellow
Cambridge Institute for Medical Research
University of Cambridge CB2 0XY
Uma's use of quotes around "di" suggests a related question about PDB
convention. It was my (perhaps not very good) understanding that ligands should be
identified by what is actually present in the crystal, and not by what can be modeled.
For example endogenous ubiquinone is likely to be UQ50
Are you sure there really are 4 chains? 50% solvent may be average, but we've
had crystals with closer to 80%.
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Pooja Kesari
[pkesar...@gmail.com]
Sent: Tuesday, January 24, 2017 10:42 PM
To: CCP4BB@
Hi Fellows,
JPK has made a valid point by reminding me that my "statement about becoming
"comfortable" with a particular level of conservatism/enthusiasm"
made in the N14 paper seems like a very non-scientific standard.
He is right. Let me explain: The difference between enthusiastic model
Dear all,Thank you very much for your replies. It is a PEG, a "di"ethylene
glycol to be precise, in most chains. Best regards,Uma. --Uma Gabale,
PhDResearch AssociateMolecular and Cellular Biochemistry
Indiana University Bloomington
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