Are you sure there really are 4 chains? 50% solvent may be average, but we've had crystals with closer to 80%.
________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Pooja Kesari [pkesar...@gmail.com] Sent: Tuesday, January 24, 2017 10:42 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Bad density for chains Dear All, I have a 2.6 A resolution structure having four chains in an asymmetric unit. The chain A and B have density for almost all residues however we don't have proper residue density in chain C and D.What can be tried to build chain C and D ? Many Thanks Pooja