Dear all,
I forgot to mention the resolution for our structure. It's 3.2 A.
-Sagar
On Wed, May 27, 2015 at 4:33 PM, Sagar De'Biomimic <
sagarbiophys...@gmail.com> wrote:
> Dear all,
>
> We have solved a structure of Protein-DNA complex. In an ASU we have two
> protein homodimers and two DNA dup
--
卢作焜
南开大学新生物站A202
在 2015-05-27 10:44:28，"Chang Qing" 写道：
Dear Eva
I checked the application information on the Web page and can only find the
information below:
Thank you for your interest in the 2015 CCP4/APS Summer School. The application
system for the 2015 school is now closed.
Dear Eva,
I still got the same information at:
http://www.ccp4.ac.uk/schools/china-2015/application.php
Thank you for your interest in the 2015 CCP4/APS Summer School. The application
system for the 2015 school is now closed. The 2016 workshop will be advertised
towards the end of 20
Hi All,
perhaps rank scaling of maps as described here
Acta Cryst. (2014). D70, 2593-2606
instead of "sigma" scaling is a way to eliminate the ambiguity inherent to
"sigma" scaling.
Pavel
On Wed, May 27, 2015 at 1:31 PM, Gerard Bricogne
wrote:
> Dear Sagar and Pavel,
>
> Yes, it may be
Dear Thierry,
This is a very interesting approach and observation. Using a
threshold of 0.6 rmsd, if converted into a ratio of B-factors, would
mean accepting waters that have a 40% higher B-factor than would be
done with a 1.0 rmsd threshold.
Philippe Dumas commented off-list on my an
University of California, San Diego
San Diego Supercomputer Center
Postdoctoral Fellow
Summary: We are looking for two highly motivated post-docs as part of our new
project “Compressive Structural Bioinformatics” funded by the US National
Institutes of Health (NIH) Big Data to Knowledge (BD2K) i
Here is another observation regarding the subject of which rms value is more
sensible to review a structure.
The purpose of the calculation described below was to determine the optimum e-
density value for rejecting waters.
The principle of the test was very simple : waters were added using th
Dear Yufan,
There was a small error on the webpage earlier and it has been fixed. So the
application window is still open.
Please try again. Thanks.
Eva
-原始邮件-
发件人: "Wu Yufan"
发送时间: 2015年5月27日 星期三
收件人: "yi...@njust.edu.cn"
抄送:
主题: 1st NJUST/CCP4 Crystallography School
Dear P
These are two different types of completness indicators
Scalepack reports Bragg's law completness, 98.8% of your unique
reflections were in diffracting condition. If you use automatic
corrections only informative reflections are output, for anisotropic
diffraction 64% is reasonable
> Hi All,
>
> S
Hi All,
Scalepack output says 98.8% complete data but after converted to .mtz file
it reduced to 64%.
I have tried in CCP4 and phenix both.
How is it possible?
Ayan
Hello Gaoyina
S. pombe riboflavin synthase has similar situation . It is homotrimeric in
solution state but the protein is monomeric in the crystal structure.
Gerhardt S, Schott AK, Kairies N, Cushman M, Illarionov B, Eisenreich W,
Bacher A, Huber R, Steinbacher S, Fischer M. Studies on the reacti
Dear Sagar and Pavel,
Yes, it may be model bias, but you also have to bear in mind that
the rmsd that is used as a unit in the choice of contouring level
should not be thought of as the standard deviation of a Gaussian
random variable and therefore implicitly associated to a "significance
lev
Sagar,
what you see may be model bias unless you calculated these maps without
that region of molecule.
Pavel
On Wed, May 27, 2015 at 12:03 PM, Sagar De'Biomimic <
sagarbiophys...@gmail.com> wrote:
> Dear all,
>
> We have solved a structure of Protein-DNA complex. In an ASU we have two
> protei
Dear All,
We are expanding our efforts on human integral membrane protein structural
biology at the SGC in Oxford and are seeking a Group Leader to build a new
group.
We are looking for a young scientist to develop a world-class programme of
research, complementing existing strengths at SG
Hi Bernhard,
Thanks for that, but it reinforces my impression (also reinforced offline by
George Sheldrick) that this is something that was floating around at some
point, and no-one ever bothered to put it in a proper publication.
The background is that we've just submitted a paper on a new way
Postdoctoral Research Position
Structural studies and inhibitors design of NHEJ pathway
A 24 month postdoctoral position is available in the Laboratory of Structural
Biology and Radiobiology. The group, led by Jean-Baptiste Charbonnier, is
located at the CEA Saclay (South Paris) and is part of t
Dear dpsk,
I asked a very similar question here about a month ago, so I'll post a
summary of the replies.
In our case, the protein was known to exist in a biologically relevant
monomer-dimer equilibrium, with the amount of monomer becoming
vanishingly small at high concentrations (e.g. in our
Dear Gaoyina,
I have not met this situation before myself, but I have some remarks:
- Are you sure that none of your crystal contacts is a dimer interface?
- Did you do SEC or SAXS at conditions close to the crystallization
conditions? Crystallization conditions themselves off
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