[ccp4bb] PISA values

2015-04-09 Thread Mohamed Noor
Dear all I have a dimer that is related to each other by a crystallographic symmetry. My questions are: a. how reliable are the delta G values given by PISA? Has there been an experimental study? b. For a dissociation delta G of say, around 30 kcal/mol, is it possible to estimate the sort of

[ccp4bb] visualizing .mrc maps in coot and model building

2015-04-09 Thread onetwo
Dear All, This is regarding the protein structure model building using the density files or the .mrc file obtained by cryo-electron microscopy data. I want to seek some help on how to visualize these files in COOT. I am working on mac system and have recently installed CCP4 which has Coot- Dec