Dear Judit
Please allow me to apologise for my recent posting on the ccp4 bulletin board
which may well have come across as slighting your own contribution. I had
earlier
made an innocent and good-natured comment on a thread that I felt was
relevant. In fact it seems to me interesting how other f
Thanks for all the good suggestions. I guess I really should try the
non-frozen data collection first.
Ursula
On Mon, Mar 2, 2015 at 11:07 AM, Keller, Jacob
wrote:
> What about 4deg data collection?
>
>
>
> What about glutaraldehyde crosslinking?
>
>
>
> JPK
>
>
>
> *From:* CCP4 bulletin board
NE-CAT at the APS wants to let you know that the deadline for the 2015-2 run is
one week away! Please get your beamtime requests in by 11:59pm March
6,2015.
https://beam.aps.anl.gov/pls/apsweb/gup0005.start_page
If you are looking for beamline please note that NE-CAT is a macromolecular
cry
Dear Colleagues,
We are writing to announce the Ultrafast X-ray Summer School 2015. UXSS 2015
will take place at DESY in Hamburg, Germany in the period from June 22, 2015 to
June 25, 2015. UXSS 2015 is jointly organized by the Center for Free-Electron
Laser Science (CFEL) at DESY and the PULSE
NE-CAT at the APS wants to let you know that the deadline for the 2015-2 run is
one week away! Please get your beamtime requests in by 11:59pm March 16,
2015.
https://beam.aps.anl.gov/pls/apsweb/gup0005.start_page
If you are looking for beamline please note that NE-CAT is a macromolecular
c
Second announcement
6th Workshop on Neutron Scattering Applications in Structural Biology
Oak Ridge, TN. June 1 - June 5, 2015
Application deadline: April 10, 2015
The workshop on Neutron Scattering Applications in Structural Biology aims at
enabling structural biologists to fully exploit the l
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Dear Ursula,
unless it is too long a list, maybe you could list the
cryo-protectants you have used so far? Did you try sugars (e.g.
glucose), or Na Malonate, or Butanediol (either 2,5 or 1,6, I don't
remember exactly)?
You could also increase the pro
An exciting Postdoc opportunity is available in Oslo, see
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Deadline: 15/3
Starting date: as soon as possible (latest 1/10)
Job description:
Postdoctoral Research Fellowship in Membrane Protein Crystallography /
CryoEM (3 years)
A postdoc
What about 4deg data collection?
What about glutaraldehyde crosslinking?
JPK
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Ursula
Schulze-Gahmen
Sent: Monday, March 02, 2015 1:49 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] cryo protection for low salt crystallization
I know there was jut recently a discussion about cryoconditions for
crystals, but I am still hoping for some new ideas for my crystals that
grow from HEPES buffer pH 7.3, 0.2 M NaCl by slowly lowering the
temperature from 20 to 4 degrees.
These crystals are easy to grow but extremely sensitive to
Dear all,
release notes of the new version are at
http://xds.mpimf-heidelberg.mpg.de/html_doc/Release_Notes.html . There are
improvements in XDS for weak data and in XSCALE for serial crystallography
applications. Furthermore, the multi-segment refinement was overhauled (mainly
affecting the I
Dear Ccp4 user,
on behalf of Dr. Muthiah Kumaraswami I would like to inform you that
there is a postdoc position available in the Department of Pathology and Genomic Medicine
at the Houston Methodist Hospital Research Institute.
The group employs a multidisciplinary approach to dissect
Dear All,
Diamond Light Source are recruiting a Senior Support Scientist for the VMXm
micro/nanofocusing MX beamline, now under construction. Details of the position
and how to apply to be found by following the link below.
http://www.diamond.ac.uk/Careers/Vacancies/All/DIA1008_CH.html
Regard
Thank you all for the guidance.
Avisek
On Mon, Mar 2, 2015 at 4:24 PM, Tim Gruene wrote:
> Dear Avisek,
>
> did you try shelxd for substructure solution? I think that it tries to
> avoid the Uranium solution. There are a couple of caveats with soaks,
> e.g. you are not sure about the number of
Postdoctoral position in Structural Biology at AstraZeneca, UK.
A postdoctoral position is currently available to work on the integrative
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Postdoctoral Research Assistant (Targeting PPIs of E3 Ubiquitin Ligases with
small molecules) - LS0568
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Full time, fixed term for 2 years in the first instance
Salary range: Grade 7 (£30,434 - £
Hi Charles,
I have some differents suggestions, maybe it could be a good idea to reconsider
the criteria for keep or discard data : Diederichs, K., et P. A. Karplus. «
Better Models by Discarding Data? ». Acta Crystallographica Section D:
Biological Crystallography 69, nᵒ 7 (1 juillet 2013): 12
Dear Avisek,
did you try shelxd for substructure solution? I think that it tries to
avoid the Uranium solution. There are a couple of caveats with soaks,
e.g. you are not sure about the number of sites and their occupancies.
Occupancies are refined by shelxd, and you can test a few number of
searc
Dear All
update 004 included a new updater for use with off line updates. This updater
is available stand alone from http://www.ccp4.ac.uk/updates/, just follow the
instructions.
Please report any bugs to c...@stfc.ac.uk.
Many thanks for using CCP4.
The CCP4 Core Team
Hi Charles,
Actually I also tried the method of Q. Liu et al and got one structure
solved after merging 3 different datasets. The only thing different in my
case is the Se-SAD phasing instead of Br. But the signal was also weak due
to the disorder of incorporated Se in the protein crystals (I supp
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