Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

Art Robbins Instruments also sells a zero-length coupler and it works well. BR From: Aaron Thompson [mailto:aaron.a.thomp...@gmail.com] Sent: Tuesday, December 16, 2014 12:02 AM To: b...@hofkristallamt.org Cc: CCP4BB@jiscmail.ac.uk Subject: Re: [ccp4bb] Fabricating hamilton syringe coupler fo

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

Hi everyone, Thanks for the advice. I had, in fact, already ordered some commercial couplers, but they haven't come in yet, and there was an experiment I wanted to do today. Here's what I found: 1. The steel ferrules have an orientation, with a "tight" side and a loose side. They can be remov

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

I agree with Jim – purchasing the couplers will get you up and running much quicker. TTP also sells nice couplers: https://www.ttplabtechstore.com/ttp_ecom/cc/ItemDetails.jsp?@where.ItemID@EQ=3072-01050&sessionkey=# Aaron On Mon, Dec 15, 2014 at 12:38 PM, Bernhard Rupp wrote: > > Tried the RN

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

Tried the RN kludge at least I did not get it to work. You cannot tighten the plastic swage lock type sleeves tight enough. On operation the pressure drives the PEEK tubing out of the compression fit. Maybe if you have a jig that holds the 2 syringes in a fixed position so they cannot move a

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

correction: www.hamiltoncompany.com addition: The compression fittings at least used to be available from us.vwr.com part number 89187-0002. On 12/15/2014 11:48 AM, Daniel Anderson wrote: Here's my addition to Jim Fairman's reply: You could use a pair of RN compression fittings (www.hamilton

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

Hi Thomas, I have been doing LCP for a while and I am using the teflon ferrules that come with the 250 uL RN syringes instead. So on one side you would have two teflon ferrules and the other side you would have the ferrule that is attached to the needle and another plastic ferrule. I have also had

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

Here's my addition to Jim Fairman's reply: You could use a pair of RN compression fittings (www.hamilton dot com part number 55751-01) and a segment of HPLC tubing. HPLC tubing within my field of view can have an inside diameter as small as 0.005 inch. hope that helps, Happy Merry, etc., Da

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

As a user of many of these coupling devices, I would highly suggest buying your couplers pre-fabricated and avoiding many hours (and possibly days) of headaches trying to manufacture these with your own hands. Rigaku Reagents sells them: https://www.rigakureagents.com/p-516-wizard-cubic-lcp-kit.as

[ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

Hi all, I'm trying to put together some homemade syringe couplers following the published instructions from the Caffrey group. I'm having a bit of trouble with this part: "The stainless steel ferrule of the second needle is removed and placed on the free end of the coupling needle such that the

Re: [ccp4bb] I am struggling to compile fasta36

Dear Pietro, zlib is the C compression library. You must have some version of libz.so installed in order to link againt it. Regards, Tim On 12/15/2014 05:36 PM, Pietro Roversi wrote: > Dear all, > > I apologise for the dullness of the question, but I am struggling to compile > the fasta36 bina

[ccp4bb] I am struggling to compile fasta36

Dear all, I apologise for the dullness of the question, but I am struggling to compile the fasta36 binary that I need to run MrBump: emmaWT:/software/fasta-36.3.7/src> sudo make -f ../make/Makefile.linux64_sse2 all gcc -g -O -msse2 -o ../bin/fasta36 comp_mthr9.o work_thr2.o pthr_subs2.o comp

[ccp4bb] Call for access to Synchrotron Beamline Facilities, 2015 - EMBL Hamburg, Germany

Call for access to Synchrotron Beamline Facilities, 2015 - EMBL Hamburg, Germany We announce a call for synchrotron beam time applications in biological small-angle X-ray scattering (SAXS) and macromolecular crystallography (MX) for the period April - November 2015. The following EMBL/DESY beaml

Re: [ccp4bb] ITC thermogram

Hi, I think the ligand concentration (250nM) is too low for the titration to reach saturation. To get properly saturated thermogram people sugest to use the ligand concentration 15-20 times higher than the macromolecule. On reducing the the ligand concentration you said you have obtained endothermi

Re: [ccp4bb] ITC thermogram

Probably, you have two mode binding between your ligand and protein. First time, when you had approximately 7 times molar excess of ligand, you could not monitor the endothermic reaction. When you went down to approx 2.5x lower conc of ligand, you could monitor endothermic reaction. However, what i